Gotoh K, Izumi H, Kanamoto T, Tamada Y, Nakashima H
Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
Biosci Biotechnol Biochem. 2000 Aug;64(8):1664-70. doi: 10.1271/bbb.64.1664.
We prepared two kinds of sulfated silk fibroins, SclFib30 and SclFib31, which contain different amounts of sulfate. These sulfated silk fibroins have anti-HIV-1 activity in vitro, apparently due to interference with the adsorption of virus particles to CD4+ cells, and completely blocked virus binding to the cells at a concentration of 100 microg/ml. Sulfated fibroins also abolished cell-to-cell infection-induced syncytium formation upon cocultivation of MOLT-4 and MOLT-4/HIV-IIIB cells, suggesting that they would interfere with gp120 and prevent the formation of gp120/CD4 complex. Silk is used in biomaterials such as surgical sutures and is believed to be a safe material for humans. In accordance with low anticoagulant activity and high anti-HIV-1 activity against both X4 HIV-1 and R5 HIV-1 strains, sulfated silk fibroins have potential as antiviral material such for a vaginal anti-HIV formulation.
我们制备了两种硫酸化丝素蛋白，即SclFib30和SclFib31，它们含有不同量的硫酸盐。这些硫酸化丝素蛋白在体外具有抗HIV-1活性，显然是由于干扰病毒颗粒与CD4+细胞的吸附，并在浓度为100微克/毫升时完全阻断病毒与细胞的结合。硫酸化丝素蛋白在MOLT-4和MOLT-4/HIV-IIIB细胞共培养时也消除了细胞间感染诱导的合胞体形成，这表明它们会干扰gp120并阻止gp120/CD4复合物的形成。丝绸被用于生物材料，如手术缝线，并且被认为是对人类安全的材料。鉴于对X4 HIV-1和R5 HIV-1毒株均具有低抗凝活性和高抗HIV-1活性，硫酸化丝素蛋白有潜力作为抗病毒材料，例如用于阴道抗HIV制剂。
