Nakashima H, Yoshida O, Tochikura T S, Yoshida T, Mimura T, Kido Y, Motoki Y, Kaneko Y, Uryu T, Yamamoto N
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Ube.
Jpn J Cancer Res. 1987 Nov;78(11):1164-8.
The inhibitory effects of several polysaccharides, dextran, xylofuranan, and ribofuranan, and their sulfated counterparts on the infectivity and replication of human immunodeficiency virus (HIV) were examined by using an HTLV-I-carrying cell line, MT-4, in vitro. Dextran sulfate (Mw 34 X 10(3], xylofuranan sulfate, and ribofuranan sulfate completely prevented HIV-induced cytopathic effects (CPE) at concentrations greater than 10 micrograms/ml and dextran sulfate (Mw 7 X 10(3] at concentrations greater than 100 micrograms/ml. However, the non-sulfated compounds did not prevent them at any concentration tested. The anti-HIV effect of these polysaccharides was confirmed by measuring HIV-specific antigen expression in infected MT-4 cells. In cocultures with MOLT-4 and MOLT-4/HIVHTLV-IIIB cells, formation of multinucleated cells was completely inhibited in the presence of 100 micrograms/ml of these sulfated compounds. Dextran sulfate showed 20-30% growth inhibition of uninfected MT-4 cells at 1000 micrograms/ml but dextran sulfate, xylofuranan sulfate, and ribofuranan sulfate showed no effect on sulfated polysaccharides efficiently inhibited the reverse transcriptase activity of avian myeloblastosis virus and HIV.
使用携带HTLV-I的细胞系MT-4在体外检测了几种多糖（葡聚糖、木糖呋喃聚糖和核糖呋喃聚糖）及其硫酸化衍生物对人类免疫缺陷病毒（HIV）感染性和复制的抑制作用。硫酸葡聚糖（Mw 34×10³）、硫酸木糖呋喃聚糖和硫酸核糖呋喃聚糖在浓度大于10微克/毫升时能完全阻止HIV诱导的细胞病变效应（CPE），硫酸葡聚糖（Mw 7×10³）在浓度大于100微克/毫升时也能完全阻止。然而，在任何测试浓度下，非硫酸化化合物都不能阻止细胞病变效应。通过检测感染的MT-4细胞中HIV特异性抗原的表达，证实了这些多糖的抗HIV作用。在与MOLT-4和MOLT-4/HIVHTLV-IIIB细胞的共培养中，在存在100微克/毫升这些硫酸化化合物的情况下，多核细胞的形成被完全抑制。硫酸葡聚糖在1000微克/毫升时对未感染的MT-4细胞有20%-30%的生长抑制作用，但硫酸葡聚糖、硫酸木糖呋喃聚糖和硫酸核糖呋喃聚糖对硫酸化多糖对禽成髓细胞瘤病毒和HIV的逆转录酶活性有有效抑制作用。 （注：原文最后一句表述稍显混乱，翻译尽量忠实原文）
