Wijaya A, Wulansari R, Ano H, Inokuma H, Makimura S
Department of Veterinary Science, Faculty of Agriculture, Miyazaki University, Japan.
J Vet Med Sci. 2000 Aug;62(8):835-9. doi: 10.1292/jvms.62.835.
In order to identify the alternative effective chemotherapeutic agents for murine babesiosis, some selected drugs were examined for their efficacy against protozoan infection in the mouse-Babesia rodhaini (B. rodhaini) model. Clindamycin was not completely effective for elimination of parasites in a dose of 50 mg or 100 mg/kg BW/day b.i.d. but effective to prolong the life span of hosts, while it completely cured B. rodhaini infections in a dose of 200 mg. On the other hand, a double therapy consisting of 2 treatments with 100 mg clindamycin and 100 mg clindamycin and with 100 mg clindamycin and 100 mg tetracycline; respectively, and a single therapy with 100 mg tetracycline or 200 mg clindamycin, had a possibility to clear away B. rodhaini organisms from hosts. However, almost all the treatment groups, had a relapse of the infection within 10 days post treatment or re-treatment. Cured mice by treatment with clindamycin and clindamycin, or clindamycin and tetracycline showed complete resistance against challenge with B. rodhaini, while mice cured by administration of clindamycin at 200 mg or tetracycline at 100 mg showed incomplete resistance to challenge infection. The present data suggest that the two former chemotherapies can induce effective protective immunity (premunization), but the latter two chemotherapies induce incomplete premunization.
为了确定用于鼠巴贝斯虫病的替代有效化疗药物，在小鼠-罗得西亚巴贝斯虫（B. rodhaini）模型中检测了一些选定药物对原生动物感染的疗效。克林霉素以50毫克或100毫克/千克体重/天的剂量每日两次给药时，对消除寄生虫并不完全有效，但对延长宿主寿命有效，而以200毫克的剂量则可完全治愈罗得西亚巴贝斯虫感染。另一方面，由100毫克克林霉素和100毫克克林霉素以及100毫克克林霉素和100毫克四环素进行两次治疗组成的联合疗法，以及100毫克四环素或200毫克克林霉素的单一疗法，有可能清除宿主体内的罗得西亚巴贝斯虫。然而，几乎所有治疗组在初次治疗或再次治疗后10天内都出现了感染复发。用克林霉素和克林霉素或克林霉素和四环素治疗治愈的小鼠对罗得西亚巴贝斯虫攻击表现出完全抗性，而用200毫克克林霉素或100毫克四环素给药治愈的小鼠对攻击感染表现出不完全抗性。目前的数据表明，前两种化疗方法可诱导有效的保护性免疫（免疫预防），而后两种化疗方法诱导的免疫预防不完全。
