Kokuho T, Hiraiwa H, Yasue H, Watanabe S, Yokomizo Y, Inumaru S
Department of Biological Product, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
J Vet Med Sci. 2000 Aug;62(8):841-7. doi: 10.1292/jvms.62.841.
Porcine genomic DNA encoding a 55 kDa subunit of interleukin-2 receptor (IL-2R), which is termed alpha chain (IL-2Ralpha), was cloned by repeated plaque hybridization using IL-2Ralpha cDNA as a probe. Two different lambda phage clones, one of which encoded exon 1 and the 5'-upstream flanking region of IL-2Ralpha gene and another encoded the sequence from exon 2 to exon 8, were isolated. By analysis of the 5'-upstream region of the gene, putative binding motifs for transcription factors such as GATA family proteins, Ikaros, NF-kappaB, NF-IL2Ralpha and SRF, were found as described in human, murine and bovine genes. Two additional motifs for STAT4 binding were also found in this region. Moreover, using the FISH technique, we assigned the porcine IL-2Ralpha locus to the distal end of the long arm of chromosome 10 (10q6-qter) where the vimentin gene had been assigned nearby.
利用白细胞介素-2受体（IL-2R）的55 kDa亚基（称为α链，即IL-2Rα）的cDNA作为探针，通过反复噬菌斑杂交克隆了编码猪基因组DNA。分离出了两个不同的λ噬菌体克隆，其中一个编码IL-2Rα基因的外显子1和5'上游侧翼区域，另一个编码从外显子2到外显子8的序列。通过对该基因5'上游区域的分析，发现了与人类、小鼠和牛基因中描述的类似的转录因子结合基序，如GATA家族蛋白、Ikaros、NF-κB、NF-IL2Rα和SRF。在该区域还发现了另外两个STAT4结合基序。此外，我们使用荧光原位杂交（FISH）技术将猪IL-2Rα基因座定位到10号染色体长臂的远端（10q6-qter），波形蛋白基因也定位在附近。
