猪白细胞介素-2受体α基因的cDNA克隆

cDNA cloning of porcine interleukin-2 receptor-alpha gene.

作者信息

Kokuho T, Uchimura A, Inumaru S

机构信息

Laboratory of Bioengineering, National Institute of Animal Health, Ibaraki, Japan.

出版信息

Immunol Cell Biol. 1997 Oct;75(5):515-8. doi: 10.1038/icb.1997.81.

DOI:10.1038/icb.1997.81

PMID:9429903

Abstract

Porcine interleukin-2 receptor-alpha subunit (IL-2R alpha) cDNA was cloned from the cDNA library of Con A-stimulated PBMC. The coding sequence of porcine IL-2R alpha, including the signal peptide sequence, is 813 b.p. in length. The identities of the sequence when it was compared with ovine, murine, feline and human sequences were 72.2, 62.4, 69.8 and 68.9% at nucleotide level and 58.9, 44.6, 54.6 and 55.6% at amino acid level, respectively. Then, the coding sequence of porcine IL-2R alpha was subcloned into the COS expression vector, pcDNA3.1/Zeo(+), and transfected into COS-7 cells. The expressed protein was specifically reactive to the mAb, 231-3B2, which seemed to be specific for porcine IL-2R alpha. This result reciprocally confirmed that the mAb, 231-3B2, recognizes porcine IL-2R alpha on a molecular basis.

摘要

猪白细胞介素-2受体α亚基（IL-2Rα）cDNA是从刀豆蛋白A刺激的外周血单核细胞（PBMC）的cDNA文库中克隆得到的。猪IL-2Rα的编码序列，包括信号肽序列，长度为813个碱基对。当该序列与绵羊、小鼠、猫和人类序列进行比较时，在核苷酸水平上的同源性分别为72.2%、62.4%、69.8%和68.9%，在氨基酸水平上的同源性分别为58.9%、44.6%、54.6%和55.6%。然后，将猪IL-2Rα的编码序列亚克隆到COS表达载体pcDNA3.1/Zeo(+)中，并转染到COS-7细胞中。表达的蛋白与单克隆抗体231-3B2具有特异性反应，该抗体似乎对猪IL-2Rα具有特异性。这一结果反过来证实了单克隆抗体231-3B2在分子水平上识别猪IL-2Rα。