Ikeda Y, Shibata I, Xuan X, Matsumoto Y, Otsuka H
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Japan.
J Vet Med Sci. 2000 Aug;62(8):849-59. doi: 10.1292/jvms.62.849.
A recombinant bovine herpesvirus type 1 (BHV-1), designated BHV-1/TF17-1, which expresses pseudorabies virus (PrV) glycoproteins gB, gC, gD, gE and gI in combination was constructed. To test the protective immunity, 10 mice were inoculated with BHV-1/TF17-1 and three weeks later 10 mice were intraperitoneally (i.p.) challenged with 20 LD50 virulent PrV (YS-81). BHV-1/TF17-1 protected all the mice from the PrV lethal challenge while all the control mice died in around 3 days. Mice vaccinated with BHV-1/TF17-1 acquired high PrV-neutralizing antibody titers and demonstrated strong delayed type hypersensitivity responses and moderate in vitro lymphocyte proliferative responses to PrV antigen. Since the major PrV glycoproteins were integrated into virions (probably into viral envelope), BHV-1/17-1 was neutralized with anti-PrV antiserum. However, the susceptibility of BHV-1/TF17-1 to anti-PrV antiserum is 2- to 4-fold lower than that of PrV vaccine lines. Our results demonstrated the possibility of BHV-1/17-1 as a vaccine to protect piglets from Audjesky's disease where maternal antibodies against PrV interfere attenuated live PrV vaccines.
构建了一种重组牛疱疹病毒1型（BHV-1），命名为BHV-1/TF17-1，其组合表达伪狂犬病病毒（PrV）糖蛋白gB、gC、gD、gE和gI。为了测试保护性免疫，10只小鼠接种BHV-1/TF17-1，三周后，10只小鼠腹腔注射20个半数致死剂量（LD50）的强毒PrV（YS-81）进行攻毒。BHV-1/TF17-1保护所有小鼠免受PrV致死性攻毒，而所有对照小鼠在约3天内死亡。接种BHV-1/TF17-1的小鼠获得了高滴度的PrV中和抗体，并表现出强烈的迟发型超敏反应以及对PrV抗原的中度体外淋巴细胞增殖反应。由于主要的PrV糖蛋白整合到病毒粒子中（可能整合到病毒包膜中），BHV-1/17-1可被抗PrV抗血清中和。然而，BHV-1/TF17-1对抗PrV抗血清的敏感性比PrV疫苗株低2至4倍。我们的结果证明了BHV-1/17-1作为一种疫苗保护仔猪免受奥杰斯基氏病侵害的可能性，在这种疾病中，针对PrV的母源抗体干扰了减毒活PrV疫苗。
