Une Y, Murakami M, Uemura K, Fujitani H, Ishibashi T, Nomura Y
Department of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
J Vet Med Sci. 2000 Aug;62(8):905-7. doi: 10.1292/jvms.62.905.
A polymerase chain reaction (PCR)-based method using a herpesvirus consensus primer was assessed for the identification of herpesviral infections in tortoises. A single band of about 230 bp was detected in PCR products from two out of twenty swabs taken from the oral cavity, three out of three paraffin-embedded tissue sections from the liver (two cases) and oral mucosa (one case), and one out of two fresh tissue samples from the oral mucosa. Nucleotide sequencing of these PCR products indicated that the herpesvirus present in these tortoises might belong to the alphaherpesvirinae. PCR using swabs and biopsy tissues was a sensitive and highly specific method for the diagnosis of herpesviral infections in tortoises.
评估了一种基于聚合酶链反应（PCR）并使用疱疹病毒通用引物的方法，用于鉴定陆龟中的疱疹病毒感染。在从口腔采集的20份拭子中的2份、来自肝脏（2例）和口腔黏膜（1例）的3份石蜡包埋组织切片中的3份以及来自口腔黏膜的2份新鲜组织样本中的1份的PCR产物中检测到一条约230 bp的单带。这些PCR产物的核苷酸测序表明，这些陆龟中存在的疱疹病毒可能属于α疱疹病毒亚科。使用拭子和活检组织进行PCR是诊断陆龟疱疹病毒感染的一种敏感且高度特异的方法。
