Une Y, Uemura K, Nakano Y, Kamiie J, Ishibashi T, Nomura Y
Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan.
Vet Pathol. 1999 Nov;36(6):624-7. doi: 10.1354/vp.36-6-624.
Large numbers of pancake tortoises (Malacochersus tornieri) and Horsfield tortoises (Testudo horsfieldii) in three consignments imported into Japan died soon after arrival. Some tortoises in the first consignment were dead on arrival. Postmortem examination of two of the pancake tortoises and four of the Horsfield tortoises revealed necrotizing lesions of the oral mucosa in both species, primarily in the tongue. Eosinophilic to amphophilic inclusion bodies were visible in the nuclei of mucosal epithelial cells in the lesions. Similar inclusion bodies were observed in the liver, spleen, adrenal glands, stomach, lungs, kidneys, small and large intestines, pancreas, and cerebrum of the pancake tortoises and in the liver, spleen, and pancreas of the Horsfield tortoises. Electron microscopic examination of the cells containing inclusion bodies showed herpesvirus-like particles about 100 nm in diameter in the cytoplasm. Nested polymerase chain reaction analysis using a herpesvirus consensus primer method confirmed the presence of a characteristic herpesvirus base sequence in tissue from these lesions.
大量进口到日本的三批扁尾陆龟（Malacochersus tornieri）和四爪陆龟（Testudo horsfieldii）在抵达后不久死亡。第一批货物中的一些陆龟在抵达时就已死亡。对两只扁尾陆龟和四只四爪陆龟进行的尸检显示，这两个物种的口腔黏膜均有坏死性病变，主要位于舌头。在病变部位的黏膜上皮细胞核中可见嗜酸性至嗜双色性的包涵体。在扁尾陆龟的肝脏、脾脏、肾上腺、胃、肺、肾脏、小肠和大肠、胰腺以及大脑中，以及在四爪陆龟的肝脏、脾脏和胰腺中观察到了类似的包涵体。对含有包涵体的细胞进行电子显微镜检查，在细胞质中发现了直径约100纳米的疱疹病毒样颗粒。使用疱疹病毒通用引物方法进行的巢式聚合酶链反应分析证实，这些病变组织中存在特征性的疱疹病毒碱基序列。
