Doyle W J, Supance J S, Marshak G, Cantekin E I, Bluestone C D, Rohn D D
Department of Otolaryngology, University of Pittsburgh School of Medicine, PA, USA.
Otolaryngol Head Neck Surg. 1982 Nov-Dec;90(6):831-6. doi: 10.1177/019459988209000627.
A chinchilla model of acute otitis media with effusion consequent to beta-lactamase-producing nontypable Haemophilus influenzae was developed using the method of direct inoculation of 145 colony-forming units (CFU) or 252 CFU of beta-lactamase-producing nontypable H influenzae into the right superior bullae of 40 chinchillas. The course of the disease was documented longitudinally by otomicroscopy, tympanometry, and periodic culturing of the middle ears. Onset of the disease occurred in 100% of the animals between two and six days postinoculation and resolution was complete in all ears by day 36. Results of rechallenge with the same organism support the combined effect of a local and weaker systemic middle ear protective mechanism rendering resistance to reinfection with a homologous organism in the chinchilla.
采用将145个菌落形成单位（CFU）或252个CFU产β-内酰胺酶的不可分型流感嗜血杆菌直接接种到40只龙猫右鼓室上隐窝的方法，建立了产β-内酰胺酶的不可分型流感嗜血杆菌所致急性分泌性中耳炎的龙猫模型。通过耳显微镜检查、鼓室导抗图检查以及中耳的定期培养对疾病进程进行纵向记录。100%的动物在接种后2至6天发病，到第36天时所有耳朵均完全恢复。用相同病原体再次攻击的结果支持了局部和较弱的全身中耳保护机制的联合作用，使龙猫对同源病原体的再感染具有抵抗力。
