Antonelli P J, Daly K A, Juhn S K, Veum E J, Adams G L, Giebink G S
Otitis Media Research Center, University of Minnesota School of Medicine, Minneapolis.
Otolaryngol Head Neck Surg. 1994 Oct;111(4):513-8. doi: 10.1177/019459989411100421.
To determine whether tobacco smoke contributes to the pathogenesis of acute otitis media, chinchillas were exposed to mainstream tobacco smoke or sham conditions (cigarettes not lit) in a Walton smoke exposure machine for 20-minute cycles two or three times daily. After 6 to 8 weeks of daily exposure, 12 chinchillas were nasally injected with Streptococcus pneumoniae, and 18 chinchillas were injected into both middle ears with nontypable Haemophilus influenzae. Smoke or sham exposures were continued for 2 to 4 weeks after injection. Otitis media developed in none of the 12 nasally injected chinchillas and in all 18 chinchillas whose middle ears were injected with nontypable Haemophilus influenzae. Persistence of middle ear effusion and persistence of nontypable Haemophilus influenzae in the middle ear effusion were not different between the smoke- and sham-exposed groups. This suggests that mainstream smoke exposure does not change the natural course of otitis media in the chinchilla model.
为了确定烟草烟雾是否会导致急性中耳炎的发病机制，将龙猫置于沃尔顿烟雾暴露机中，每天进行2至3次20分钟的循环，使其暴露于主流烟草烟雾或假暴露条件（香烟未点燃）下。在每天暴露6至8周后，给12只龙猫经鼻注射肺炎链球菌，给18只龙猫的双耳注射不可分型流感嗜血杆菌。注射后继续进行烟雾或假暴露2至4周。经鼻注射的12只龙猫均未发生中耳炎，而所有18只中耳注射了不可分型流感嗜血杆菌的龙猫均发生了中耳炎。中耳积液的持续时间以及中耳积液中不可分型流感嗜血杆菌的持续存在情况在烟雾暴露组和假暴露组之间并无差异。这表明在龙猫模型中，主流烟雾暴露不会改变中耳炎的自然病程。
