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[基于线粒体DNA的PCR-RFLP分析推断亚洲和北美多丽红点鲑(Salvelinus malma)的分化]

[Differentiation of Dolly Varden char Salvelinus malma from Asia and North America inferred from PCR-RFLP analysis of mitochondrial DNA].

作者信息

Oleĭnik A G, Skurikhina L A, Brykov V A, Crane P A, Wenburg J K

出版信息

Genetika. 2005 May;41(5):626-34.

PMID:15977814
Abstract

Genetic differentiation of Dolly Varden char Salvelinus malma Walbaum from the Asian and North American Pacific coasts was studied. We examined restriction fragment length polymorphism of three mitochondrial DNA (mtDNA) fragments amplified in polymerase chain reaction, which encoded four NADH dehydrogenase subunits, the cytochrome b gene, and a D-loop segment. The mtDNA haplotypes were shown to form three phylogenetic groups, whose geographic distribution corresponded to three Dolly Varden subspecies: S. malma malma, S. malma krascheninnikovi, and S. malma lordi. The nucleotide sequence divergence between S. malma malma and S. malma krascheninnikovi was 3.8%; between S. malma malma and S. malma lordi, 3.1%; and between S. malma krascheninnikovi and S. malma lordi, 2.5%. The northern Dolly Varden S. malma malma from Asia was shown to be genetically identical to that from North America.

摘要

对来自亚洲和北美太平洋沿岸的花羔红点鲑(Salvelinus malma Walbaum)的遗传分化进行了研究。我们检测了在聚合酶链反应中扩增的三个线粒体DNA(mtDNA)片段的限制性片段长度多态性,这些片段编码四个NADH脱氢酶亚基、细胞色素b基因和一个D环区段。结果表明,mtDNA单倍型形成了三个系统发育组,其地理分布对应于三个花羔红点鲑亚种:S. malma malma、S. malma krascheninnikovi和S. malma lordi。S. malma malma与S. malma krascheninnikovi之间的核苷酸序列差异为3.8%;S. malma malma与S. malma lordi之间为3.1%;S. malma krascheninnikovi与S. malma lordi之间为2.5%。结果显示,来自亚洲的北部花羔红点鲑S. malma malma与来自北美的在基因上是相同的。

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