tRNA前体的体外合成及其向成熟大小tRNA的转化。

In vitro synthesis of tRNA precursors and their conversion to mature size tRNA.

作者信息

Daniel V, Grimberg J I, Zeevi M

出版信息

Nature. 1975 Sep 18;257(5523):193-7. doi: 10.1038/257193a0.

Abstract

Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+ and su3-) and tRNA2Tyr, tRNA2Gly (su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.

摘要

两个大肠杆菌tRNA基因簇，即tRNA1Tyr（su3+和su3-）以及tRNA2Tyr、tRNA2Gly（su+36）、tRNA3Thr，在一个纯化的体外系统中进行转录。证据表明，相邻的tRNA基因作为一个大尺寸的共同前体一起转录，该前体与粗细胞提取物一起温育时，会产生成熟的tRNA分子。