Knapp G, Beckmann J S, Johnson P F, Fuhrman S A, Abelson J
Cell. 1978 Jun;14(2):221-36. doi: 10.1016/0092-8674(78)90109-5.
Genes for yeast tRNATyr and tRNAPhe have been sequenced (Goodman, Olson and Hall, 1977; Valenzuela et al., 1978) which contain additional nucleotides (intervening sequences) within the middle of the gene that are not present in the mature tRNA. We have isolated precursors to rRNATyr and tRNAPhe from a yeast temperature-sensitive mutant (at the rna1 locus) which accumulates only certain precursor tRNAs at the nonpermissive temperature. The tRNATyr and tRNAPhe precursors were analyzed by oligonucleotide mapping; they each contain the intervening sequence and fully matured 5' and 3' termini. Furthermore, these precursors were used as substrates to search for an enzymatic activity which can remove the intervening sequences and religate the ends. We have shown that wild-type yeast contains such an activity, and that this activity specifically removes the intervening sequences to produce mature-sized RNAs.
酵母tRNATyr和tRNAPhe的基因已被测序（古德曼、奥尔森和霍尔，1977年；巴伦苏埃拉等人，1978年），这些基因在基因中部含有额外的核苷酸（间隔序列），而这些核苷酸在成熟的tRNA中并不存在。我们从一个酵母温度敏感突变体（rna1位点）中分离出了rRNATyr和tRNAPhe的前体，该突变体在非允许温度下仅积累某些前体tRNA。通过寡核苷酸图谱分析了tRNATyr和tRNAPhe前体；它们各自都包含间隔序列以及完全成熟的5'和3'末端。此外，这些前体被用作底物来寻找一种能够去除间隔序列并连接末端的酶活性。我们已经表明，野生型酵母含有这种活性，并且这种活性能够特异性地去除间隔序列以产生成熟大小的RNA。
