Polonio R E, Wolke R E, MacLean S A, Sperry J F
Department of Fisheries, Animal & Veterinary Sciences, University of Rhode Island, Kingston 02881, USA.
Fish Shellfish Immunol. 2000 Jul;10(5):405-18. doi: 10.1006/fsim.1999.0248.
An in vitro phagocytosis assay was developed for hybrid striped bass (Morone saxatilis x Morone chrysops), using cells collected from the peritoneal cavity of this fish. The findings indicated that: (1) 10 days following a single intraperitoneal injection (1 ml) of Freund's incomplete adjuvant (FIA) was an appropriate time for collecting suitable working concentrations (5.3+/-4.0 x 10(7) cells ml(-1)) of peritoneal phagocytes (83.7+/-1.5% macrophages) from these hybrids held at 23 degrees C; (2) these cells phagocytosed latex beads (polystyrene microspheres 3.12 microm in diameter) after 30 min of in vitro incubation at room temperature (25+/-1 degrees C). The phagocytic ability and phagocytic capacity in a washed adherent layer exposure system were 67.2+/-2.76% and 4.14+/-0.35 beads phagocyte(-1), respectively. These results strongly suggest that a simple methodology, including baseline data serving as guidelines, is now available for conducting in vitro phagocytosis assays in this hybrid.
利用从杂交条纹鲈（条纹鲈×金眼鲈）腹腔收集的细胞，开发了一种体外吞噬试验。研究结果表明：（1）在23℃饲养的这些杂交鱼中，单次腹腔注射（1毫升）弗氏不完全佐剂（FIA）10天后，是收集合适工作浓度（5.3±4.0×10⁷个细胞/毫升）腹腔吞噬细胞（83.7±1.5%为巨噬细胞）的适宜时间；（2）在室温（25±1℃）下体外孵育30分钟后，这些细胞吞噬了乳胶珠（直径3.12微米的聚苯乙烯微球）。在洗涤后的贴壁层暴露系统中，吞噬能力和吞噬容量分别为67.2±2.76%和4.14±0.35个珠子/吞噬细胞。这些结果有力地表明，现在有一种简单的方法，包括作为指导方针的基线数据，可用于在这种杂交鱼中进行体外吞噬试验。
