用从红花黄色花瓣中纯化得到的一种酶将前红花苷酶促转化为红花苷。

Enzymatic conversion of precarthamin to carthamin by a purified enzyme from the yellow petals of safflower.

作者信息

Cho M H, Paik Y S, Hahn T R

机构信息

Departments of Genetic Engineering and Chemistry, Plant Metabolism Research Center, Kyung Hee University, Suwon, 449-701, Korea.

出版信息

J Agric Food Chem. 2000 Sep;48(9):3917-21. doi: 10.1021/jf9911038.

DOI:10.1021/jf9911038

PMID:10995291

Abstract

Precarthamin, a yellow precursor of carthamin, was efficiently isolated from the yellow petals of safflower (Carthamus tinctorius L. ) with Sephadex LH-20 column chromatography and preparative HPLC, and identified with UV-vis and NMR spectrometry. The UV-vis spectrum of precarthamin showed lambda(max) of 238 and 406 nm in MeOH. The molar extinction coefficients of precarthamin at 406 nm in MeOH and 50 mM citrate buffer (pH 5.0) were 59 000 M(-)(1) cm(-)(1) and 45 400 M(-)(1) cm(-)(1), respectively. The isolated and structurally identified precarthamin was converted to a red pigment by a homogeneously purified enzyme from the immature petals of safflower in 50 mM citrate buffer (pH 5.0). The enzymatically converted red pigment was identified as carthamin, a red pigment of safflower by TLC, HPLC, and UV-vis spectral analysis.

摘要

红花黄色素（一种红花红素的黄色前体）通过葡聚糖凝胶LH - 20柱色谱和制备型高效液相色谱从红花（Carthamus tinctorius L.）的黄色花瓣中高效分离出来，并通过紫外 - 可见光谱和核磁共振光谱进行鉴定。红花黄色素在甲醇中的紫外 - 可见光谱显示其最大吸收波长（λ(max)）为238和406 nm。红花黄色素在甲醇中以及在50 mM柠檬酸盐缓冲液（pH 5.0）中406 nm处的摩尔消光系数分别为59000 M⁻¹ cm⁻¹和45400 M⁻¹ cm⁻¹。在50 mM柠檬酸盐缓冲液（pH 5.0）中，从红花未成熟花瓣中纯化得到的一种酶可将分离并经结构鉴定的红花黄色素转化为一种红色色素。通过薄层色谱法（TLC）、高效液相色谱法（HPLC）和紫外 - 可见光谱分析，经酶促转化得到的红色色素被鉴定为红花红素，即红花的一种红色色素。