Sato K, Sugimoto N, Ohta M, Yamazaki T, Maitani T, Tanamoto K
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Food Addit Contam. 2003 Nov;20(11):1015-22. doi: 10.1080/02652030310001615177.
Carthamin is a well-known major pigment in carthamus red colourant. When analysed by HPLC on an ODS column, the colorant separated into two distinct reddish pigments, with both components having almost identical photodiode array spectra. LC/MS analysis suggested one of the compounds was carthamin, whilst the other was an unknown minor pigment. The minor pigment was purified and isolated from the colorant by preparative LC/MS collecting the faction based on monitoring the deprotonated molecule M-H m/z 953 in electrospray negative-ion mode. The structure was elucidated as a hydroxyethyl ether of carthamin, a novel compound, by means of NMR and HR-FAB-MS analyses.
红花苷是红花红色素中一种著名的主要色素。当在ODS柱上通过高效液相色谱法进行分析时，该色素分离成两种不同的红色素，两种成分具有几乎相同的光电二极管阵列光谱。液相色谱/质谱分析表明其中一种化合物是红花苷，而另一种是未知的次要色素。通过制备型液相色谱/质谱从色素中纯化并分离出次要色素，在电喷雾负离子模式下基于监测去质子化分子M-H m/z 953收集馏分。通过核磁共振和高分辨快原子轰击质谱分析，其结构被阐明为红花苷的羟乙基醚，一种新型化合物。
