Ruaro M E, Stebel M, Vatta P, Marzinotto S, Schneider C
Laboratorio Nazionale Consorzio Interuniversitario per le Bitecnologie, Area Science Park, Padriciano 99, 34012 Trieste, Italy.
FEBS Lett. 2000 Sep 15;481(2):159-63. doi: 10.1016/s0014-5793(00)02005-6.
The product of the growth arrest specific gene, gas1, is a membrane-associated protein which activates a p53-dependent growth suppression signalling pathway. We have shown that Gas1 is linked to the plasma membrane through a glycosyl-phosphatidylinositol (GPI) anchor. Several GPI-anchored protein have been identified as part of receptor complexes either as co-receptors or as membrane bound ligands. In this report, we characterize the Gas1 domains required for its growth suppression function and demonstrate the dispensability of Gas1 GPI anchor.
生长停滞特异性基因gas1的产物是一种膜相关蛋白，它激活一条依赖p53的生长抑制信号通路。我们已经表明Gas1通过糖基磷脂酰肌醇（GPI）锚定与质膜相连。几种GPI锚定蛋白已被鉴定为受体复合物的一部分，要么作为共受体，要么作为膜结合配体。在本报告中，我们描述了Gas1生长抑制功能所需的结构域，并证明了Gas1的GPI锚定是不必要的。
