Ulcova-Gallova Z, Krauz V, Rokyta Z
Department of Obstetrics and Gynecology, Faculty Hospital, Charles University, Pilsen, Czech Republic.
Int J Fertil Womens Med. 2000 Jul-Aug;45(4):292-6.
Determination of aPLs in ovulatory mucus and in seminal plasma in 18 infertile couples (women, median age 33.2, range 28-39 years; men, median age 36.4, range 29-46 years) with repeated unexplained miscarriages (four to nine spontaneous abortions). Women with high levels of aPLs in sera were excluded from the study.
aPL screening included cardiolipin (Sevac Prague), L-alpha-phosphatidic acid, L-alpha-phosphatidylethanolamine, L-alpha-phosphatidyl-DL-glycerol, L-alpha-phosphatidylinositol, and L-alpha-phosphatidyl/serine (Sigma, USA). These were determined in isotypes IgG and IgA by an ELISA method. Statistical analysis of aPL results was made by cut-off levels of aPLs for both Ig isotypes by using 3 SD or 95th percentile calculated by STATGRAPHICS. Control group was created from four fertile healthy couples.
Levels of aPLs in ovulatory cervical mucus were in the majority very low, except for IgA-aPLs-inositol. Very high interindividual differences in aPLs were found in seminal plasma. IgA-aPL-ethanolamine and -DL-glycerol, and IgG-aPL-L-serine and -DL-glycerol predominated in seminal plasma. Extremely high IgA levels of aPLs in all above-mentioned antigens, except cardiolipin, were found in one man of an infertile couple.
The passage of abnormal aPLs levels from seminal plasma into the site of conception during often repeated, unprotected intercourses is supported by the findings.
测定18对不育夫妇（女性，中位年龄33.2岁，范围28 - 39岁；男性，中位年龄36.4岁，范围29 - 46岁）排卵黏液和精浆中的抗磷脂抗体（aPLs），这些夫妇有反复不明原因流产史（4至9次自然流产）。血清中aPLs水平高的女性被排除在研究之外。
aPLs筛查包括心磷脂（捷克塞瓦克公司）、L-α-磷脂酸、L-α-磷脂酰乙醇胺、L-α-磷脂酰-DL-甘油、L-α-磷脂酰肌醇和L-α-磷脂酰/丝氨酸（美国西格玛公司）。通过酶联免疫吸附测定法测定这些物质的IgG和IgA同种型。使用STATGRAPHICS计算的3个标准差或第95百分位数作为两种Ig同种型aPLs的临界值，对aPLs结果进行统计分析。对照组由4对生育健康的夫妇组成。
除IgA-aPL-肌醇外，排卵宫颈黏液中的aPLs水平大多非常低。精浆中aPLs存在非常高的个体差异。精浆中以IgA-aPL-乙醇胺和-DL-甘油以及IgG-aPL-L-丝氨酸和-DL-甘油为主。在一对不育夫妇的一名男性中，发现上述所有抗原（除心磷脂外）的aPLs的IgA水平极高。
这些发现支持了在频繁的无保护性交过程中，异常水平的aPLs从精浆进入受孕部位的观点。
