Kapoor A, Talib V H, Verma S K
Department of Laboratory Medicine, Safdarjung Hospital, New Delhi.
Indian J Pathol Microbiol. 1999 Jan;42(1):37-43.
160 clinical samples were collected from 40 infertile couples with unexplained infertility. The samples collected included serum and seminal plasma of the male partners and serum and cervical mucus samples of the female partners. 25 fertile healthy couples were investigated as controls. All the samples collected were then tested for class-specific antisperm antibodies by an Enzyme linked immunosorbent assay (ELISA). Antisperm antibodies were detected in 30% of the infertile couples which included 25% female and 10% male partners. Amongst the cases positive for antisperm antibodies, antibodies were detected most frequently in female sera 58.4% followed by male sera 33% and 25% in cervical mucus. The isotyping of antisperm antibodies in various samples showed IgG to be the most frequent type specific antibody followed by IgM & IgA types of antibodies. ELISA has provided a relatively simple, reliable and highly reproducible method of detection of antisperm antibodies. Thus application of antisperm antibody testing especially in cervical mucus should become an integral part of the investigation of immunologic infertility.
从40对不明原因不孕的不育夫妇中收集了160份临床样本。收集的样本包括男性伴侣的血清和精浆以及女性伴侣的血清和宫颈黏液样本。对25对有生育能力的健康夫妇进行了调查作为对照。然后通过酶联免疫吸附测定（ELISA）对所有收集的样本进行特异性抗精子抗体检测。在30%的不育夫妇中检测到抗精子抗体，其中包括25%的女性伴侣和10%的男性伴侣。在抗精子抗体阳性的病例中，女性血清中抗体检测频率最高，为58.4%，其次是男性血清中的33%和宫颈黏液中的25%。各种样本中抗精子抗体的分型显示IgG是最常见的特异性抗体类型，其次是IgM和IgA类型的抗体。ELISA提供了一种相对简单、可靠且高度可重复的抗精子抗体检测方法。因此，抗精子抗体检测的应用，尤其是在宫颈黏液中的检测，应成为免疫性不孕调查的一个组成部分。
