Milewicz A, Mikulski E, Bidzińska B
Department of Endocrinology and Diabetology, Medical University of Wroclaw, Poland.
Int J Obes Relat Metab Disord. 2000 Jun;24 Suppl 2:S152-3. doi: 10.1038/sj.ijo.0801310.
Obesity is an important factor predisposing to type 2 diabetes mellitus, especially for postmenopausal women. Experimental studies provided evidences that leptin, cholecystokinin (CCK), galanin (GAL), neuropeptide Y (NPY) and insulin are involved in feeding behaviour. The aim of the study was to evaluate their possible relationships in obese and diabetic women. Three groups of postmenopausal women (FSH > 30 mIU/ml) were evaluated: 8 diabetic (mean age 56.6 +/- 6.9 y, BMI 29.8 +/- 5.3 kg/m2), 10 obese non-diabetic (mean age 49.6 +/- 5.4 y, BMI 36.0 +/- 3.7 kg/m2) and 12 non-diabetic controls (mean age 52.7 +/- 3.5 y, BMI 27.3 1.9 kg/m2). For each patient BMI and WHR were measured and calculated. Blood samples were collected at 8:00 a.m. after an overnight fast. Plasma concentrations of FSH, leptin, CCK, GAL, NPY and insulin were determined using commercial RIA kits. Mean plasma NPY concentration was significantly higher in diabetic women than in controls (190.1 pg/ml +/- 85.4 vs 120.4 +/- 36.6). Compared to controls, mean plasma leptin level was significantly higher in obese non-diabetic women (32.9ng/ml +/- 9.2 vs 18.9 +/- 9.1). No significant differences were found between obese non-diabetic and diabetic women. In diabetic subjects positive correlations were found between: CCK and leptin (r= 0.8295; P= 0.011), CCK and insulin (r=0.7832; P=0.022), leptin and insulin (r=0.9302; P=0.001). In obese subjects a positive correlation between WHR and GAL (r= 0.6624; P= 0.037) and a negative between GAL and insulin (r= -0.6795; P= 0.031) were found. In controls positive correlations were found between WHR and CCK (r=0.6412; P=0.025), GAL and insulin (r=0.630; P=0.028) and negative between CCK and NPY (r = -0.6505; P= 0.022). Our results, ie higher mean plasma NPY levels in postmenopausal diabetic women and positive correlation of CCK with leptin and insulin, may suggest the role of these neuropeptides in metabolic disorders leading to type 2 diabetes mellitus.
肥胖是诱发2型糖尿病的一个重要因素，对绝经后女性而言尤其如此。实验研究表明，瘦素、胆囊收缩素（CCK）、甘丙肽（GAL）、神经肽Y（NPY）和胰岛素与进食行为有关。本研究的目的是评估它们在肥胖和糖尿病女性中的可能关系。对三组绝经后女性（促卵泡激素>30 mIU/ml）进行了评估：8名糖尿病患者（平均年龄56.6±6.9岁，体重指数29.8±5.3 kg/m²），10名肥胖非糖尿病患者（平均年龄49.6±5.4岁，体重指数36.0±3.7 kg/m²）和12名非糖尿病对照者（平均年龄52.7±3.5岁，体重指数27.3±1.9 kg/m²）。测量并计算了每位患者的体重指数和腰臀比。在禁食过夜后于上午8:00采集血样。使用商用放射免疫分析试剂盒测定血浆中促卵泡激素、瘦素、胆囊收缩素、甘丙肽、神经肽Y和胰岛素的浓度。糖尿病女性的血浆NPY平均浓度显著高于对照组（190.1 pg/ml±85.4 vs 120.4±36.6）。与对照组相比，肥胖非糖尿病女性的血浆瘦素平均水平显著更高（32.9ng/ml±9.2 vs 18.9±9.1）。肥胖非糖尿病女性和糖尿病女性之间未发现显著差异。在糖尿病受试者中发现：胆囊收缩素与瘦素之间呈正相关（r = 0.8295；P = 0.011），胆囊收缩素与胰岛素之间呈正相关（r = 0.7832；P = 0.022），瘦素与胰岛素之间呈正相关（r = 0.9302；P = 0.001）。在肥胖受试者中发现腰臀比与甘丙肽之间呈正相关（r = 0.6624；P = 0.037），甘丙肽与胰岛素之间呈负相关（r = -0.6795；P = 0.031）。在对照组中发现腰臀比与胆囊收缩素之间呈正相关（r = 0.6412；P = 0.025），甘丙肽与胰岛素之间呈正相关（r = 0.630；P = 0.028），胆囊收缩素与神经肽Y之间呈负相关（r = -0.6505；P = 0.022）。我们的研究结果，即绝经后糖尿病女性的血浆NPY平均水平较高以及胆囊收缩素与瘦素和胰岛素呈正相关，可能提示这些神经肽在导致2型糖尿病的代谢紊乱中所起的作用。
