Joksimovic Jovana, Selakovic Dragica, Jovicic Nemanja, Mitrovic Slobodanka, Mihailovic Vladimir, Katanic Jelena, Milovanovic Dragan, Rosic Gvozden
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Department of Histology and Embryology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Front Neurosci. 2019 Feb 26;13:172. doi: 10.3389/fnins.2019.00172. eCollection 2019.
The aim of our study was to evaluate the effects of chronic administration of nandrolone-decanoate (ND) or testosterone-enanthate (TE) in supraphysiological doses and a prolonged swimming protocol, alone and in combination with ND or TE, on anxiety-like behavior in rats. We investigated the immunohistochemical alterations of the hippocampal neuropeptide Y (NPY) and melanocortin 4 receptor (MC4R) neurons, as a possible underlying mechanism in a modulation of anxiety-like behavior in rats. Both applied anabolic androgenic steroids (AASs) induced anxiogenic effect accompanied with decreased serum and hippocampal NPY. The exercise-induced anxiolytic effect was associated with increased hippocampal NPY expression. ND and TE increased the number of MC4R, while the swimming protocol was followed by the reduction of MC4R in the CA1 region of the hippocampus. However, NPY/MC4R ratio in hippocampus was lowered by AASs and elevated by exercise in all hippocampal regions. An augmentation of this ratio strongly and positively correlated to increased time in open arms of elevated plus maze, in the context that indicates anxiolytic effect. Our findings support the conclusion that alterations in both hippocampal NPY and MC4R expression are involved in anxiety level changes in rats, while their quantitative relationship (NPY/MC4R ratio) is even more valuable in the estimation of anxiety regulation than individual alterations for both NPY and MC4R expression in the hippocampus.
我们研究的目的是评估超生理剂量长期给予癸酸诺龙（ND）或庚酸睾酮（TE）以及延长游泳方案单独或与ND或TE联合使用对大鼠焦虑样行为的影响。我们研究了海马神经肽Y（NPY）和黑皮质素4受体（MC4R）神经元的免疫组化改变，作为调节大鼠焦虑样行为的一种可能潜在机制。两种应用的合成代谢雄激素类固醇（AASs）均诱导焦虑效应，同时血清和海马NPY降低。运动诱导的抗焦虑效应与海马NPY表达增加有关。ND和TE增加了MC4R的数量，而游泳方案后海马CA1区的MC4R减少。然而，AASs降低了海马各区域的NPY/MC4R比值，而运动则提高了该比值。在表明抗焦虑效应的情况下，该比值的增加与高架十字迷宫开放臂停留时间的增加呈强烈正相关。我们的研究结果支持以下结论：海马NPY和MC4R表达的改变均参与大鼠焦虑水平的变化，而它们的定量关系（NPY/MC4R比值）在评估焦虑调节方面比海马中NPY和MC4R表达的个体改变更有价值。
