Berry J E, Murphy C M, Smith G A, Ranasinghe E, Finberg R, Walton J, Brown J, Navarrete C, Metcalfe P, Ouwehand W H
Division of Haematology, National Institute for Biological Standards and Control, Potters Bar, UK.
Br J Haematol. 2000 Sep;110(3):735-42. doi: 10.1046/j.1365-2141.2000.02170.x.
The glycosylphosphatidylinositol-linked platelet protein CD109 carries the biallelic alloantigen system Gov. There is limited information on the incidence of Gov alloantibodies in neonatal alloimmune thrombocytopenia (NAITP), post-transfusion purpura (PTP) and platelet refractoriness. We adapted the monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay to the detection of Gov antibodies and determined their incidence in 605 archived samples (112 with HPA antibodies) referred for the aforementioned conditions. Here, we show that CD109 expression was reduced upon platelet storage in saline or by cryopreservation, but was stable when stored as whole blood or therapeutic platelet concentrate. Fourteen of the 605 samples contained Gov alloantibodies (anti-Gova, n = 10; anti-Govb, n = 4), with the majority in platelet refractoriness (n = 9) and, of the remaining five, four in NAITP and one in PTP. In seven cases, no other HPA antibodies were detected, three being NAITP cases. The incidence of Gov antibodies was significantly lower than HPA-1 system antibodies (n = 87), but equalled the number of HPA-5 system antibodies (n = 14) and outnumbered HPA-2 and -3 system antibodies (10 altogether).
糖基磷脂酰肌醇连接的血小板蛋白CD109携带双等位基因同种抗原系统Gov。关于Gov同种抗体在新生儿同种免疫性血小板减少症（NAITP）、输血后紫癜（PTP）和血小板输注无效中的发生率的信息有限。我们将血小板抗原的单克隆抗体特异性固定化（MAIPA）检测方法应用于Gov抗体的检测，并在因上述情况送检的605份存档样本（112份含有血小板同种抗原（HPA）抗体）中确定了它们的发生率。在此，我们表明，血小板在盐水中储存或冷冻保存后，CD109表达降低，但作为全血或治疗性血小板浓缩物储存时则保持稳定。605份样本中有14份含有Gov同种抗体（抗Gov a，n = 10；抗Gov b，n = 4），其中大多数出现在血小板输注无效病例中（n = 9），其余5份中，4份出现在NAITP中，1份出现在PTP中。在7例病例中，未检测到其他HPA抗体，其中3例为NAITP病例。Gov抗体的发生率显著低于HPA-1系统抗体（n = 87），但与HPA-5系统抗体的数量相当（n = 14），且多于HPA-2和-3系统抗体（共10份）。
