Bastard J P, Jardel C, Bruckert E, Blondy P, Capeau J, Laville M, Vidal H, Hainque B
Service de Biochimie, Hôpital de la Salpêtrière, Paris, France.
J Clin Endocrinol Metab. 2000 Sep;85(9):3338-42. doi: 10.1210/jcem.85.9.6839.
The aim of this study was to investigate the potential role of adipose cytokines in the obesity-associated insulin resistance. To that end, we compared: 1) serum concentrations of interleukin 6 (IL-6), tumor necrosis factor alpha (TNFalpha), and leptin in eight healthy lean control females and in android obese female without (n = 14) and with (n = 7) type 2 diabetes; and 2) the levels of these cytokines both in serum and in sc adipose tissue in the 14 obese nondiabetic women before and after 3 weeks of a very low-calorie diet (VLCD). As compared with lean controls, obese nondiabetic and diabetic patients were more insulin resistant and presented increased values for leptin, IL-6, TNFalpha, and C-reactive protein. In the whole group, IL-6 values were more closely related to the parameters evaluating insulin resistance than leptin or TNFalpha values. VLCD resulted in weight loss and decreased body fat mass (approximately 3 kg). Insulin sensitivity was improved with no significant change in both serum and adipose tissue TNFalpha levels. In contrast, VLCD induced significant decreases in IL-6 and leptin levels in both adipose tissue and serum. These results suggest that, as for leptin, circulating IL-6 concentrations reflect, at least in part, adipose tissue production. The reduced production and serum concentrations after weight loss could play a role in the improved sensitivity to insulin observed in these patients.
本研究的目的是调查脂肪细胞因子在肥胖相关胰岛素抵抗中的潜在作用。为此，我们进行了以下比较：1）8名健康瘦素对照女性、14名无2型糖尿病的腹型肥胖女性和7名有2型糖尿病的腹型肥胖女性的血清白细胞介素6（IL-6）、肿瘤坏死因子α（TNFα）和瘦素浓度；2）14名肥胖非糖尿病女性在极低热量饮食（VLCD）3周前后血清和皮下脂肪组织中这些细胞因子的水平。与瘦素对照相比，肥胖非糖尿病和糖尿病患者的胰岛素抵抗更强，瘦素、IL-6、TNFα和C反应蛋白的值升高。在整个组中，IL-6值与评估胰岛素抵抗的参数比瘦素或TNFα值更密切相关。VLCD导致体重减轻和体脂量减少（约3kg）。胰岛素敏感性得到改善，血清和脂肪组织TNFα水平无显著变化。相反，VLCD导致脂肪组织和血清中IL-6和瘦素水平显著降低。这些结果表明，与瘦素一样，循环IL-6浓度至少部分反映了脂肪组织的产生。体重减轻后产生减少和血清浓度降低可能在这些患者观察到的胰岛素敏感性改善中起作用。
