儿童脑肿瘤的体外化学敏感性：对洛莫司汀（CCNU）和长春新碱敏感性差异的证据

Chemosensitivity in childhood brain tumours in vitro: evidence of differential sensitivity to lomustine (CCNU) and vincristine.

作者信息

Lewandowicz G M, Harding B, Harkness W, Hayward R, Thomas D G, Darling J L

机构信息

University Department of Neurosurgery, Institute of Neurology, University College, National Hospital for Neurology and Neurosurgery, Queen Square, London WC1N 3BG, UK.

出版信息

Eur J Cancer. 2000 Oct;36(15):1955-64. doi: 10.1016/s0959-8049(00)00245-8.

DOI:10.1016/s0959-8049(00)00245-8

PMID:11000577

Abstract

The aim of this study was to examine the range of sensitivity of a panel of short-term cultures derived from different types of malignant childhood brain tumours including medulloblastoma, ependymoma and glioblastoma multiforme to three cytotoxic drugs, lomustine (CCNU), vincristine (VCR) and procarbazine (PCB). Sensitivity was assessed using a modification of the dimethylthiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay. Short-term cell lines derived from ependymomas were considerably more resistant to VCR than other types of childhood brain tumours, while cultures derived from supratentorial primitive neuroectodermal tumour (PNET) displayed marked sensitivity to both lomustine and VCR. Cultures from ependymomas, medulloblastoma and astrocytic gliomas had similar sensitivity to lomustine and PCB as cultures derived from adult malignant astrocytoma.

摘要

本研究的目的是检测一组源自不同类型儿童恶性脑肿瘤（包括髓母细胞瘤、室管膜瘤和多形性胶质母细胞瘤）的短期培养物对三种细胞毒性药物洛莫司汀（CCNU）、长春新碱（VCR）和丙卡巴肼（PCB）的敏感性范围。使用改良的噻唑蓝（MTT）法评估敏感性。源自室管膜瘤的短期细胞系对VCR的耐药性明显高于其他类型的儿童脑肿瘤，而源自幕上原始神经外胚层肿瘤（PNET）的培养物对洛莫司汀和VCR均表现出显著敏感性。来自室管膜瘤、髓母细胞瘤和星形胶质细胞瘤的培养物对洛莫司汀和PCB的敏感性与源自成人恶性星形细胞瘤的培养物相似。