Sakaki T, Kitagawa S, Tsuda T
Department of Applied Chemistry, Nagoya Institute of Technology, Showa, Gokiso, Japan.
Electrophoresis. 2000 Sep;21(15):3088-92. doi: 10.1002/1522-2683(20000901)21:15<3088::AID-ELPS3088>3.0.CO;2-M.
The instrumentation for miniaturization of capillary electrochromatography was devised and an injection method for this apparatus was proposed. By using an ultra short capillary column (15 mm packed length, 36 mm total length, 75 microm inner diameter, packed with cation exchange supports), the separation of five biochemical compounds was performed within 1 min. The high separation efficiency of 9780 plates was achieved by using an ultrashort capillary column. The miniaturized instrumentation for capillary electrochromatography might be utilized as one of the possible methods in microfabricated analysis or in an alternative approach to it.
设计了用于毛细管电色谱小型化的仪器，并提出了该仪器的进样方法。通过使用超短毛细管柱（填充长度15 mm，总长度36 mm，内径75μm，填充阳离子交换载体），在1分钟内完成了5种生化化合物的分离。使用超短毛细管柱实现了9780理论塔板数的高分离效率。毛细管电色谱的小型化仪器可作为微制造分析或其替代方法中可能的方法之一。
