Slentz B E, Penner N A, Lugowska E, Regnier F
Department of Chemistry, Purdue University, West Lafayette, IN 47907-1393, USA.
Electrophoresis. 2001 Oct;22(17):3736-43. doi: 10.1002/1522-2683(200109)22:17<3736::AID-ELPS3736>3.0.CO;2-Y.
Following current trends in miniaturization of analytical chemistry, an inexpensive disposable analytical tool in the form of a liquid chromatography column fabricated on a poly(dimethyl siloxane) (PDMS) chip was created. Ease of fabricating the chromatography column was demonstrated by molding collocated monolithic support structures (COMOSS) directly in the column. Positive photo-resist, SPR 220, was used to create column structures on a negative relief master providing final channel dimensions of 2.7-5.2 microm wide by 10.0 microm deep, while monolithic dimensions were 9.8 x 9.8 x 10.0 microm - 12.3 x 12.3 x 10.0 microm. The ability to separate biological samples such as peptides from a tryptic digest of fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) was shown. Separations in capillary electrochromatographic (CEC) mode were performed yielding column efficiencies of 4.0 x 10(5) plates/m.
遵循分析化学小型化的当前趋势，制造出了一种廉价的一次性分析工具，它是一种在聚二甲基硅氧烷（PDMS）芯片上制作的液相色谱柱形式。通过在柱中直接模制并置的整体支撑结构（COMOSS）证明了制备色谱柱的简便性。使用正性光刻胶SPR 220在负性浮雕母版上创建柱结构，最终通道尺寸为宽2.7 - 5.2微米、深10.0微米，而整体尺寸为9.8×9.8×10.0微米 - 12.3×12.3×10.0微米。展示了从异硫氰酸荧光素标记的牛血清白蛋白（FITC - BSA）的胰蛋白酶消化物中分离生物样品（如肽）的能力。以毛细管电色谱（CEC）模式进行分离，柱效为4.0×10⁵塔板/米。
