Breadmore M C, Haddad P R, Fritz J S
University of Tasmania, Hobart, Australia.
Electrophoresis. 2000 Sep;21(15):3181-90. doi: 10.1002/1522-2683(20000901)21:15<3181::AID-ELPS3181>3.0.CO;2-L.
The influence of varying experimental conditions on the mobilities of inorganic and organic anions in ion chromatography-capillary electrophoresis (IC-CE) was studied. A theoretical model derived from both IC and CE was used to explain the influence on analyte mobility caused by varying the concentration of polymer and increasing the salt concentration in the background electrolyte. The influence of the type of competing ion was also accounted for by including the analyte selectivity coefficient in the model equation. The validity of the model was shown using electrolyte systems containing four different competing anions, with correlation between experimental and mobilities predicted being excellent (r2 > 0.98) for all systems. Selectivity coefficients determined via nonlinear regression enabled quantitative comparisons of different competing ion strength, with the eluting strength increasing in the order of fluoride, acetate, chloride, and sulfate. Optimization of the polymer and eluent concentration was performed for all electrolyte systems using the normalized resolution product optimization criterion, requiring only seven experiments to obtain the optimum conditions for complete separation. The minimum resolution criterion was used to optimize the fluoride system which gave a different separation selectivity from both CE and IC.
研究了不同实验条件对离子色谱 - 毛细管电泳（IC - CE）中无机和有机阴离子迁移率的影响。采用源自离子色谱和毛细管电泳的理论模型来解释通过改变聚合物浓度和增加背景电解质中盐浓度对分析物迁移率的影响。通过在模型方程中纳入分析物选择性系数，也考虑了竞争离子类型的影响。使用包含四种不同竞争阴离子的电解质系统展示了该模型的有效性，所有系统的实验迁移率与预测迁移率之间的相关性都非常好（r2 > 0.98）。通过非线性回归确定的选择性系数能够对不同竞争离子强度进行定量比较，洗脱强度按氟化物、乙酸盐、氯化物和硫酸盐的顺序增加。使用归一化分离度乘积优化标准对所有电解质系统进行聚合物和洗脱液浓度的优化，仅需七次实验即可获得完全分离的最佳条件。使用最小分离度标准对氟化物系统进行优化，该系统给出了与毛细管电泳和离子色谱不同的分离选择性。
