Wallenborg S R, Lurie I S, Arnold D W, Bailey C G
Sandia National Laboratories, Livermore, CA 94551-0969, USA.
Electrophoresis. 2000 Sep;21(15):3257-63. doi: 10.1002/1522-2683(20000901)21:15<3257::AID-ELPS3257>3.0.CO;2-M.
Amphetamine and analogous compounds have been labeled with 4-fluoro-7-nitrobenzofurazane and analyzed on a microfabricated chip. Separation of norephedrine, ephedrine, cathinone, pseudoephedrine, methcathinone, amphetamine and methamphetamine is demonstrated using micellar electrokinetic capillary chromatography (MEKC) and laser-induced fluorescence (LIF) detection. Chiral separations of individual drugs were studied using neutral and negatively charged cyclodextrins (CDs) with and without the addition of an organic modifier and/or sodium dodecyl sulfate (SDS). The best results were obtained using a highly sulfated gamma-CD (HS-gamm-CD) in combination with a low concentration of SDS. To obtain complete separation of a mixture of (+/-)-norephedrine, (+/-)ephedrine, (+/-)-pseudoephedrine, (+/-)-methcathinone, (+/-)-amphetamine and (+/-)-methamphetamine it was necessary to add a small amount (1.5 mM) of SDS to the separation buffer. Optimized chiral separation was achieved within 7 min using an S-folded separation channel, a separation voltage of 8 kV and a buffer consisting of 50 mM phosphate (pH 7.35), 10 mM HS-gamma-CD and 1.5 mM SDS.
苯丙胺及类似化合物已用4-氟-7-硝基苯并呋喃标记，并在微制造芯片上进行分析。使用胶束电动毛细管色谱法（MEKC）和激光诱导荧光（LIF）检测，实现了去甲麻黄碱、麻黄碱、卡西酮、伪麻黄碱、甲卡西酮、苯丙胺和甲基苯丙胺的分离。使用带中性和负电荷的环糊精（CDs），在添加和不添加有机改性剂和/或十二烷基硫酸钠（SDS）的情况下，研究了各药物的手性分离。使用高度硫酸化的γ-环糊精（HS-γ-CD）与低浓度的SDS相结合，获得了最佳结果。为了实现（±）-去甲麻黄碱、（±）-麻黄碱、（±）-伪麻黄碱、（±）-甲卡西酮、（±）-苯丙胺和（±）-甲基苯丙胺混合物的完全分离，有必要在分离缓冲液中添加少量（1.5 mM）的SDS。使用S形分离通道、8 kV的分离电压以及由50 mM磷酸盐（pH 7.35）、10 mM HS-γ-CD和1.5 mM SDS组成的缓冲液，在7分钟内实现了优化的手性分离。
