Shihabi Z K
Pathology Department, Wake Forest University Baptist Medical Center, Winston-Salem, NC 27157, USA.
Electrophoresis. 2000 Aug;21(14):2872-8. doi: 10.1002/1522-2683(20000801)21:14<2872::AID-ELPS2872>3.0.CO;2-G.
Discontinuous buffers for capillary zone electrophoresis (CZE) can be used under less rigid conditions compared to those for isotachophoresis for stacking. They can be prepared simply by modifying the sample itself, either by addition of small inorganic ions, low conductivity diluents, or both, and also by adjusting its pH, meanwhile injecting a large volume on the capillary. Zwitterionic and organic-based buffers such as triethanolamine and tris(hydroxymethyl)aminomethane (Tris) are well suited for stacking due to their low conductivity, provided the buffer is discontinuous as demonstrated here. A simple mechanism based on discontinuous buffers is described to explain many of the observed stacking types in CZE, pointing out the many similarities to transient isotachophoresis.
与等速电泳堆积所用的缓冲液相比，毛细管区带电泳（CZE）的不连续缓冲液可在较宽松的条件下使用。它们可以通过简单地改变样品本身来制备，方法是添加小的无机离子、低电导率稀释剂或两者兼而有之，同时调节其pH值，并在毛细管上进样大量样品。两性离子和有机类缓冲液，如三乙醇胺和三（羟甲基）氨基甲烷（Tris），由于其低电导率，非常适合堆积，前提是此处所证明的缓冲液是不连续的。描述了一种基于不连续缓冲液的简单机制，以解释CZE中观察到的许多堆积类型，指出了与瞬态等速电泳的许多相似之处。
