Shihabi Z K
Pathology Department, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
J Capillary Electrophor. 1995 Nov-Dec;2(6):267-71.
A new type of sample stacking for capillary zone electrophoresis (CZE), which occurs in mixtures of acetonitrile and high concentrations of inorganic salts in the sample, is described here. It is different from the stacking obtained in low-ionic-strength buffers in the sample since it is improved by the presence of a high concentration of ions while the latter type deteriorates in the presence of such ions. It is based on the addition of acetonitrile to the sample to eliminate proteins by precipitation. From a practical point of view, the stacking in acetonitrile is more useful since it both removes proteins and counteracts the deleterious effects of high concentrations of inorganic ions present in the sample. Furthermore, it allows a larger volume of the sample to be loaded on the capillary. More than one-third of the capillary can be loaded with the sample by this type of stacking, producing a high sample concentration with good resolution and increased sensitivity of detection. It is well suited for analyzing small molecules in serum, urine, food, tissues, and industrial samples. Furthermore, since proteins are eliminated, the capillary does not need thorough washings between samples, speeding up the analysis and extending the capillary life.
本文描述了一种用于毛细管区带电泳（CZE）的新型样品堆积方法，该方法发生在样品中的乙腈和高浓度无机盐的混合物中。它与在低离子强度缓冲液中获得的堆积不同，因为它在高浓度离子存在下得到改善，而后者在这种离子存在下会变差。它基于向样品中添加乙腈以通过沉淀去除蛋白质。从实际角度来看，乙腈中的堆积更有用，因为它既能去除蛋白质，又能抵消样品中高浓度无机离子的有害影响。此外，它允许在毛细管上加载更大体积的样品。通过这种类型的堆积，可以将超过三分之一的毛细管加载样品，产生高样品浓度，具有良好的分辨率和提高的检测灵敏度。它非常适合分析血清、尿液、食物、组织和工业样品中的小分子。此外，由于蛋白质被去除，样品之间毛细管不需要彻底清洗，从而加快了分析速度并延长了毛细管寿命。
