注射合成促黄体生成素释放因子（LRF）后大鼠垂体促性腺细胞的超微结构
Ultrastructure of rat pituitary gonadotrophs after injection of synthetic luteinizing hormone releasing factor (LRF).
作者信息
Nakano R, Lee W, Hayashi K, Tojo S
出版信息
Endokrinologie. 1975 Feb;65(1):60-5.
The effects of intracarotid injection of synthetic luteinizing hormone releasing factor (LRF) on the ultrastructure of pituitary gonadotrophs are reported. Chronically ovariectomized rats of the Sprague-Dawley strain pretreated with oestradiol and progesterone were sacrificed at zero time and at 30, 120 min after injection of 1 mug of synthetic LRF. A careful observation was made for evidence of granule extrusion from gonadotrophs following intracarotid injection of LRF, but no signs of granule extrusion were demonstrated. However, granule migration to the periphery of the cell was observed 30 min after LRF injection and the distribution of secretory granules recovered 2 hours after injection. The result of ultrastructural observations suggests that LRF plays an important role in releasing and synthesizing gonadotrophins in pituitary gonadotrophs. Further investigations should elucidate more clearly the morphologic aspect of mode of LRF action in pituitary gonadotrophs.
本文报道了颈内注射合成促黄体生成激素释放因子（LRF）对垂体促性腺细胞超微结构的影响。选用经雌激素和孕激素预处理的慢性去卵巢斯普拉格-道利大鼠，在注射1微克合成LRF后的0分钟、30分钟和120分钟处死。仔细观察颈内注射LRF后促性腺细胞颗粒排出的证据，但未发现颗粒排出的迹象。然而，在注射LRF后30分钟观察到颗粒向细胞周边迁移，且在注射2小时后分泌颗粒的分布恢复。超微结构观察结果表明，LRF在垂体促性腺细胞中促性腺激素的释放和合成中起重要作用。进一步的研究应更清楚地阐明LRF在垂体促性腺细胞中的作用方式的形态学方面。
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