Lecanidou R, Richards E G
Eur J Biochem. 1975 Sep 1;57(1):127-33. doi: 10.1111/j.1432-1033.1975.tb02283.x.
Solutions of native, renatured and denatured 5-S RNA from Escherichia coli were each found to revert to a mixture of the denatured and renatured forms when incubated at elevated temperatures in the absence of divalent cations. The equilibrium and rate constants for the renaturation and denaturation reactions were studied as a function of temperature and concentration of added NaCl. The enthalpy and entropy of denaturation and the activation energies were determined. The activation energies for the renaturation reaction were found to be almost independent of the salt concentration, whereas the other parameters increased with the salt concentration. The results are interpreted in terms of a partially unfolded intermediate whose concentration is suppressed by the addition of salt.
研究发现，来自大肠杆菌的天然、复性和变性5-S RNA溶液，在没有二价阳离子的情况下于高温孵育时，每种都会回复到变性和复性形式的混合物。研究了复性和变性反应的平衡常数和速率常数与温度及添加NaCl浓度的函数关系。测定了变性的焓、熵以及活化能。发现复性反应的活化能几乎与盐浓度无关，而其他参数则随盐浓度增加。结果用一种部分展开的中间体来解释，其浓度会因添加盐而受到抑制。
