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核磁共振（NMR）证据表明5S核糖核酸存在两种天然构象。

NMR evidence for the existence of two native conformations of 5S RNA.

作者信息

Kime M J, Moore P B

出版信息

Nucleic Acids Res. 1982 Aug 25;10(16):4973-83. doi: 10.1093/nar/10.16.4973.

DOI:10.1093/nar/10.16.4973
PMID:6182528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320845/
Abstract

NMR spectra of the non-exchangeable protons in 5S RNA from E. coli show the existence of two distinct conformers of the molecule which meet the operational definition of "A form" or native 5S RNA. Both are easily distinguished spectroscopically from denatured, "B form" 5S RNA. The conditions which interconvert the two A form conformers strongly suggest that the transition between them gives rise to the low temperature optical melting transition first reported in 5S RNA by Kao and Crothers (1).

摘要

大肠杆菌5S RNA中不可交换质子的核磁共振光谱显示，该分子存在两种不同的构象异构体，它们符合“A型”或天然5S RNA的操作定义。两者在光谱上都很容易与变性的“B型”5S RNA区分开来。使两种A型构象异构体相互转化的条件强烈表明，它们之间的转变导致了Kao和Crothers（1）首次报道的5S RNA低温光学熔解转变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be98/320845/153aa68139b3/nar00385-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be98/320845/c95a382cb8ed/nar00385-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be98/320845/153aa68139b3/nar00385-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be98/320845/c95a382cb8ed/nar00385-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be98/320845/153aa68139b3/nar00385-0157-a.jpg

相似文献

1
NMR evidence for the existence of two native conformations of 5S RNA.核磁共振（NMR）证据表明5S核糖核酸存在两种天然构象。
Nucleic Acids Res. 1982 Aug 25;10(16):4973-83. doi: 10.1093/nar/10.16.4973.
2
Does 5S RNA from E. coli have a pseudoknotted structure?来自大肠杆菌的5S RNA是否具有假结结构？
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6
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Hydrogen-1 and phosphorus-31 nuclear magnetic resonance study of the solution structure of Bacillus licheniformis 5S ribonucleic acid.地衣芽孢杆菌5S核糖核酸溶液结构的氢-1和磷-31核磁共振研究
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Secondary structure of 5S RNA: NMR experiments on RNA molecules partially labeled with nitrogen-15.5S核糖核酸的二级结构：对部分用氮-15标记的核糖核酸分子进行的核磁共振实验。
Biochemistry. 1987 Aug 11;26(16):5213-20. doi: 10.1021/bi00390a047.

引用本文的文献

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2
Comparative structural analysis of eubacterial 5S rRNA by oxidation of adenines in the N-1 position.通过对N-1位腺嘌呤进行氧化对真细菌5S核糖体RNA进行比较结构分析。
Nucleic Acids Res. 1984 Apr 11;12(7):3115-26. doi: 10.1093/nar/12.7.3115.
3
Oligonucleotide directed mutagenesis of Escherichia coli 5S ribosomal RNA: construction of mutant and structural analysis.

本文引用的文献

1
A proton NMR study of ribosomal protein L25 from Escherichia coli.来自大肠杆菌的核糖体蛋白L25的质子核磁共振研究。
Eur J Biochem. 1981 May 15;116(2):269-76. doi: 10.1111/j.1432-1033.1981.tb05329.x.
2
A hydrogen exchange study of Escherichia coli 5S RNA.大肠杆菌5S核糖核酸的氢交换研究。
Nucleic Acids Res. 1981 Aug 25;9(16):4081-6. doi: 10.1093/nar/9.16.4081.
3
A proton-coupled conformational switch of Escherichia coli 5S ribosomal RNA.大肠杆菌5S核糖体RNA的质子偶联构象开关
大肠杆菌5S核糖体RNA的寡核苷酸定向诱变：突变体构建及结构分析
Nucleic Acids Res. 1984 Sep 25;12(18):6935-50. doi: 10.1093/nar/12.18.6935.
4
Defining the binding site of Xenopus transcription factor IIIA on 5S RNA using truncated and chimeric 5S RNA molecules.利用截短和嵌合的5S RNA分子确定非洲爪蟾转录因子IIIA在5S RNA上的结合位点。
Nucleic Acids Res. 1987 Mar 25;15(6):2737-55. doi: 10.1093/nar/15.6.2737.
5
A comparison of the solution structures and conformational properties of the somatic and oocyte 5S rRNAs of Xenopus laevis.非洲爪蟾体细胞和卵母细胞5S rRNA的溶液结构及构象性质比较
Nucleic Acids Res. 1988 Mar 25;16(5):2295-312. doi: 10.1093/nar/16.5.2295.
6
Does 5S RNA from E. coli have a pseudoknotted structure?来自大肠杆菌的5S RNA是否具有假结结构？
Nucleic Acids Res. 1986 Sep 25;14(18):7473-85. doi: 10.1093/nar/14.18.7473.
7
Characterization of the RNA binding properties of transcription factor IIIA of Xenopus laevis oocytes.非洲爪蟾卵母细胞转录因子IIIA的RNA结合特性表征
Nucleic Acids Res. 1985 Jul 25;13(14):5369-87. doi: 10.1093/nar/13.14.5369.
8
Comparative calorimetric studies on the dynamic conformation of plant 5S rRNA: II. Structural interpretation of the thermal unfolding patterns for lupin seeds and wheat germ.植物5S rRNA动态构象的比较量热研究：II. 羽扇豆种子和小麦胚芽热解链模式的结构解释
Nucleic Acids Res. 1991 May 11;19(9):2449-55. doi: 10.1093/nar/19.9.2449.
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3360-4. doi: 10.1073/pnas.77.6.3360.
4
Rearrangement of the conformation of Escherichia coli 5S RNA.大肠杆菌5S RNA构象的重排
Proc Natl Acad Sci U S A. 1968 Sep;61(1):292-9. doi: 10.1073/pnas.61.1.292.
5
Simple method for the characterization of 5S RNA.5S核糖核酸特性鉴定的简易方法。
Anal Biochem. 1972 Jan;45(1):319-25. doi: 10.1016/0003-2697(72)90035-8.
6
The kinetics of renaturation of 5-S RNA from Escherichia coli in the presence of Mg 2+ ions.在镁离子存在的情况下，大肠杆菌5-S RNA的复性动力学。
Eur J Biochem. 1973 Apr;34(2):262-7. doi: 10.1111/j.1432-1033.1973.tb02755.x.
7
Size and shape of 5 S ribosomal RNA.5S核糖体RNA的大小和形状。
J Mol Biol. 1972 Oct 28;71(1):31-7. doi: 10.1016/0022-2836(72)90398-1.
8
The thermodynamics and kinetics of conformational changes in 5-S RNA from Escherichia coli.来自大肠杆菌的5-S RNA构象变化的热力学和动力学
Eur J Biochem. 1975 Sep 1;57(1):127-33. doi: 10.1111/j.1432-1033.1975.tb02283.x.
9
Molecular model for 5-S RNA. A small-angle x-ray scattering study of native, denatured and aggregated 5-S RNA from Escherichia coli ribosomes.5-S RNA的分子模型。对来自大肠杆菌核糖体的天然、变性和聚集的5-S RNA的小角X射线散射研究。
Eur J Biochem. 1976 Sep 15;68(2):481-7. doi: 10.1111/j.1432-1033.1976.tb10835.x.
10
Role of 5S RNA in assembly and function of the 50S subunit from Escherichia coli.5S RNA在大肠杆菌50S亚基组装及功能中的作用
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2221-5. doi: 10.1073/pnas.73.7.2221.