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dam或seqA基因的无效突变可抑制温度敏感致死性，但不能抑制muk无效突变体对新生霉素的超敏感性。

Null mutation of the dam or seqA gene suppresses temperature-sensitive lethality but not hypersensitivity to novobiocin of muk null mutants.

作者信息

Onogi T, Yamazoe M, Ichinose C, Niki H, Hiraga S

机构信息

Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862-0976, Japan.

出版信息

J Bacteriol. 2000 Oct;182(20):5898-901. doi: 10.1128/JB.182.20.5898-5901.2000.

DOI:10.1128/JB.182.20.5898-5901.2000
PMID:11004192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC94715/
Abstract

Escherichia coli mukF, mukE, and mukB null mutants have common phenotypes such as temperature-dependent colony formation, anucleate cell production, chromosome cutting by septum closure, and abnormal localization of SeqA-DNA clusters. We show here that the associated muk null mutations cause hypersensitivity to novobiocin. Null mutation of either dam or seqA suppressed partially the temperature-sensitive lethality but failed to suppress the anucleate cell production and the hypersensitivity to novobiocin caused by muk null mutations.

摘要

大肠杆菌mukF、mukE和mukB基因敲除突变体具有共同的表型，如温度依赖性菌落形成、无核细胞产生、隔膜闭合导致的染色体切割以及SeqA-DNA簇的异常定位。我们在此表明，相关的muk基因敲除突变会导致对新生霉素高度敏感。dam或seqA的基因敲除突变可部分抑制温度敏感性致死性，但无法抑制muk基因敲除突变导致的无核细胞产生和对新生霉素的高度敏感性。

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J Bacteriol. 2000 Oct;182(20):5898-901. doi: 10.1128/JB.182.20.5898-5901.2000.
2
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本文引用的文献

1
Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli.大肠杆菌中SeqA结合的半甲基化DNA簇的双向迁移及oriC拷贝的配对
Genes Cells. 2000 May;5(5):327-41. doi: 10.1046/j.1365-2443.2000.00334.x.
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Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I.通过拓扑异构酶I中的突变抑制大肠杆菌muk突变体的染色体分离缺陷
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1671-6. doi: 10.1073/pnas.030528397.
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Dynamic organization of chromosomal DNA in Escherichia coli.大肠杆菌中染色体DNA的动态组织
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Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli.参与大肠杆菌染色体分离的MukB、MukE和MukF蛋白的复合物形成
EMBO J. 1999 Nov 1;18(21):5873-84. doi: 10.1093/emboj/18.21.5873.
5
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Mol Microbiol. 1999 Oct;34(1):157-68. doi: 10.1046/j.1365-2958.1999.01589.x.
6
The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli.SeqA-Gfp融合蛋白在大肠杆菌活细胞中的组装与迁移。
Mol Microbiol. 1999 Mar;31(6):1775-82. doi: 10.1046/j.1365-2958.1999.01313.x.
7
Binding of SeqA protein to DNA requires interaction between two or more complexes bound to separate hemimethylated GATC sequences.SeqA蛋白与DNA的结合需要两个或更多结合在不同半甲基化GATC序列上的复合物之间相互作用。
EMBO J. 1999 Apr 15;18(8):2304-10. doi: 10.1093/emboj/18.8.2304.
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J Struct Biol. 1998 Dec 15;124(2-3):303-10. doi: 10.1006/jsbi.1998.4056.
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DNA gyrase and topoisomerase IV: biochemical activities, physiological roles during chromosome replication, and drug sensitivities.DNA 回旋酶和拓扑异构酶IV：生化活性、染色体复制过程中的生理作用以及药物敏感性。
Biochim Biophys Acta. 1998 Oct 1;1400(1-3):29-43. doi: 10.1016/s0167-4781(98)00126-2.
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FEBS Lett. 1998 Jul 3;430(3):278-82. doi: 10.1016/s0014-5793(98)00677-2.