Levine C, Hiasa H, Marians K J
Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, NY, USA.
Biochim Biophys Acta. 1998 Oct 1;1400(1-3):29-43. doi: 10.1016/s0167-4781(98)00126-2.
DNA gyrase and topoisomerase IV are the two type II topoisomerases present in bacteria. Though clearly related, based on amino acid sequence similarity, they each play crucial, but distinct, roles in the cell. Gyrase is involved primarily in supporting nascent chain elongation during replication of the chromosome, whereas topoisomerase IV separates the topologically linked daughter chromosomes during the terminal stage of DNA replication. These different roles can be attributed to differences in the biochemical properties of the two enzymes. The biochemical activities, physiological roles, and drug sensitivities of the enzymes are reviewed.
DNA 回旋酶和拓扑异构酶IV是细菌中存在的两种II型拓扑异构酶。尽管基于氨基酸序列相似性它们明显相关，但它们在细胞中各自发挥着关键但不同的作用。回旋酶主要参与染色体复制过程中新生链的延伸，而拓扑异构酶IV在DNA复制的末期将拓扑连接的子代染色体分开。这些不同的作用可归因于这两种酶生化特性的差异。本文综述了这些酶的生化活性、生理作用和药物敏感性。
