Negrusz A, Moore C M, Stockham T L, Poiser K R, Kern J L, Palaparthy R, Le N L, Janicak P G, Levy N A
Department of Pharmaceutics and Pharmacodynamics, College of Pharmacy, University of Illinois at Chicago, 60612, USA.
J Forensic Sci. 2000 Sep;45(5):1031-40.
The hypnotic benzodiazepine flunitrazepam (Rohypnol) has been identified as the drug of choice for the purposes of "drugging" unsuspecting victims and raping them while they are under the influence of this substance. The objective of this paper was to study elimination of flunitrazepam and 7-aminoflunitrazepam in urine collected from ten healthy volunteers who received a single 2 mg oral dose of Rohypnol, to determine how long after drug administration 7-aminoflunitrazepam can be detected. A highly sensitive NCI-GC-MS method for the simultaneous quantitation of flunitrazepam (LOQ 100 pg/mL) and 7-aminoflunitrazepam (LOQ 10 pg/mL) in urine was developed. All samples were screened for benzodiazepines using optimized micro-plate enzyme immunoassay. The highest concentrations of 7-aminoflunitrazepam (70-518 ng/mL) and flunitrazepam (0.7-2.8 ng/mL) in urine were observed 6 h after drug administration in nine subjects and after 24 h in one subject. In six subjects 7-aminoflunitrazepam was detected up to 14 days after flunitrazepam administration, in one subject up to 21 days and in three subjects up to 28 days. In urine samples collected from six volunteers, flunitrazepam was detected three days after Rohypnol intake, in three subjects 24 h, and in one subject 5 days later. Benzodiazepine micro-plate enzyme immunoassay kit allowed the detection of flunitrazepam and metabolities 5 to 21 days after drug administration.
催眠性苯二氮䓬类药物氟硝西泮（“迷奸药”）已被确认为“迷奸”毫无防备的受害者并在她们受该药物影响时实施强奸的首选药物。本文的目的是研究10名健康志愿者单次口服2毫克氟硝西泮后尿液中氟硝西泮和7-氨基氟硝西泮的消除情况，以确定给药后多久可以检测到7-氨基氟硝西泮。建立了一种高灵敏度的NCI-GC-MS方法，用于同时定量尿液中的氟硝西泮（定量下限100 pg/mL）和7-氨基氟硝西泮（定量下限10 pg/mL）。使用优化的微孔板酶免疫测定法对所有样品进行苯二氮䓬类药物筛查。9名受试者在给药后6小时、1名受试者在给药后24小时观察到尿液中7-氨基氟硝西泮（70-518 ng/mL）和氟硝西泮（0.7-2.8 ng/mL）的最高浓度。在6名受试者中，在氟硝西泮给药后14天内检测到7-氨基氟硝西泮，1名受试者中检测到21天，3名受试者中检测到28天。在6名志愿者采集的尿液样本中，氟硝西泮在服用氟硝西泮后3天被检测到，3名受试者在24小时后被检测到，1名受试者在5天后被检测到。苯二氮䓬类微孔板酶免疫测定试剂盒可在给药后5至21天检测到氟硝西泮及其代谢物。
