Wu Yu-Hong, Tan Jia-Yi, Xia Yong
Department of Forensic Medicine, Criminal Police College of China, Shenyang 110035, China.
Se Pu. 2002 Mar;20(2):182-4.
A rapid and highly sensitive method, based on high performance thin-layer chromatography, is described for the qualitative and semiquantitative determination of 7-aminoflunitrazepam, the main metabolite of flunitrazepam in human urine. 7-Aminoflunitrazepam in specimen was extracted by solid phase extraction using GDX-403 porous polymer bead as sorbent and ethyl ether as eluant. Fluorescamine was used as a reagent to produce fluorescent product on the plate. The limit of detection was 5 micrograms/L and the limit of quantitation was 15 micrograms/L for 7-aminoflunitrazepam in urine. The method can be successfully used for measuring 7-aminoflunitrazepam in urine samples of the subjects excreted over a 48 h period after receiving 1 mg flunitrazepam orally. The method is applicable to drug examination for the cases of drug-facilitated robbery and rape.
本文描述了一种基于高效薄层色谱法的快速、高灵敏度方法，用于定性和半定量测定人体尿液中氟硝西泮的主要代谢物7-氨基氟硝西泮。尿样中的7-氨基氟硝西泮采用GDX-403多孔聚合物微球作为吸附剂、乙醚作为洗脱剂的固相萃取法进行提取。使用荧光胺作为试剂在薄层板上产生荧光产物。尿液中7-氨基氟硝西泮的检测限为5微克/升，定量限为15微克/升。该方法可成功用于测定口服1毫克氟硝西泮后48小时内排泄的受试者尿样中的7-氨基氟硝西泮。该方法适用于药物辅助抢劫和强奸案件的毒品检测。
