Riches P E, Everitt N M, McNally D S
Department of Anatomy, University of Bristol, UK.
J Biomech. 2000 Dec;33(12):1551-7. doi: 10.1016/s0021-9290(00)00148-2.
The Knoop indenter has been used to characterise fully the Knoop microhardness (H(K)) anisotropy of compact bone. 2120 indentations were performed on mature ovine radii and a linear relationship was found between H(K) and the angle between the major diagonal of the indenter and the lamella boundaries (p<<0.001). H(K) increased significantly with ash fraction (p<0.001), but decreased with atmospheric vapour pressure (p<0.05). A significant interaction was found between ash fraction and atmospheric vapour pressure (p<0.01). H(K) significantly varied with indentation position along the diaphysis and around the cortex (both p<<0.001), however radial variation in H(K) was not statistically significant. The variation of ash fraction showed similar trends. These data show that H(K) varies similarly to Vickers microhardness, but in addition, can provide clear information on the anisotropy of Haversian bone without the need for excising many different indentation planes. A large number of indentations are required to obtain low type I and type II errors in the statistical analysis.
努氏压头已被用于全面表征致密骨的努氏显微硬度（H(K)）各向异性。在成熟的绵羊桡骨上进行了2120次压痕试验，发现H(K)与压头主对角线和骨板边界之间的夹角呈线性关系（p<<0.001）。H(K)随灰分含量显著增加（p<0.001），但随大气蒸气压降低（p<0.05）。发现灰分含量与大气蒸气压之间存在显著交互作用（p<0.01）。H(K)沿骨干和皮质周围的压痕位置有显著变化（两者p<<0.001），然而H(K)的径向变化无统计学意义。灰分含量的变化呈现类似趋势。这些数据表明，H(K)的变化与维氏显微硬度相似，但此外，无需切割许多不同的压痕平面就能提供有关哈弗斯骨各向异性的清晰信息。在统计分析中需要大量压痕以获得低的I型和II型误差。
