Nakayama H, Kitayama J, Muto T, Nagawa H
Department of Surgery, The University of Tokyo, Japan.
Jpn J Clin Oncol. 2000 Jul;30(7):301-5. doi: 10.1093/jjco/hyd078.
Analysis of serum cytokine levels has shown that cancer-bearing hosts have lower levels of IL-2 and IFN-gamma, suggesting that Th1-type immunity is impaired by cancer. However, the mechanisms of the Th1 dysfunction are not clearly understood.
The frequencies of Th1 cells in CD4(+) helper T cells were evaluated with an intracytoplasmic cytokine staining method in peripheral blood lymphocytes (PBL) and lymph node lymphocytes (LNL) of patients with gastrointestinal cancer.
Activation of lymphocytes with PMA + Ionomycin induced the expression of IL-2 and IFN-gamma in each lymphocyte population. Compared with PBL of non-malignant donors, PBL in cancer patients contained significantly lower frequencies of CD4(+) T cells that produced IL-2 and IFN-gamma. LNL in cancer patients also contained lower levels of IL-2- and IFN-gamma-producing CD4(+) T cells, although the percentages did not show significant differences from those of PBL in the same patients.
Our data suggest that suppression of Th1 cytokine in cancer patients is, at least in part, due to the decreased frequency of Th1 cells with CD4(+) phenotype.
血清细胞因子水平分析表明，患癌宿主的白细胞介素-2（IL-2）和γ干扰素（IFN-γ）水平较低，这表明癌症会损害Th1型免疫。然而，Th1功能障碍的机制尚不清楚。
采用胞内细胞因子染色法评估胃肠道癌患者外周血淋巴细胞（PBL）和淋巴结淋巴细胞（LNL）中CD4(+)辅助性T细胞中Th1细胞的频率。
用佛波酯（PMA）+离子霉素激活淋巴细胞可诱导各淋巴细胞群体中IL-2和IFN-γ的表达。与非恶性供体的PBL相比，癌症患者的PBL中产生IL-2和IFN-γ的CD4(+) T细胞频率显著降低。癌症患者的LNL中产生IL-2和IFN-γ的CD4(+) T细胞水平也较低，尽管其百分比与同一患者PBL的百分比相比无显著差异。
我们的数据表明，癌症患者中Th1细胞因子的抑制至少部分是由于具有CD4(+)表型的Th1细胞频率降低所致。
