日本口腔鳞状细胞癌患者中人类乳头瘤病毒16和18感染及p53突变的发生率

Incidence of human papillomavirus 16 and 18 infection and p53 mutation in patients with oral squamous cell carcinoma in Japan.

作者信息

Shima K, Kobayashi I, Saito I, Kiyoshima T, Matsuo K, Ozeki S, Ohishi M, Sakai H

机构信息

First Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.

出版信息

Br J Oral Maxillofac Surg. 2000 Oct;38(5):445-50. doi: 10.1054/bjom.2000.0162.

DOI:10.1054/bjom.2000.0162

PMID:11010772

Abstract

The purpose of this study was to investigate the prevalence of human papillomaviruses (HPVs) 16 and 18 infection, and p53 mutation in oral squamous cell carcinomas (SCCs) in Japanese patients. Our results showed a higher incidence of HPV16 and 18 infections than previous studies because we combined the findings of a consensus polymerase chain reaction (PCR), restriction fragment length polymorphism by using the restriction enzyme digestion of the PCR products and Southern blot hybridization. Each HPV16 and 18 E6/E7 DNA was detected in 9 (20%) and 25 (54%) of 46 samples. The p53 mutation in the exons from 5 to 8 were detected in 20 out of 46 samples (43%) by a PCR-single strand conformation polymorphism analysis. There was a significant relationship between HPV16 and the p53 mutation (P =0.02) suggesting that HPV16 infection has a mutagenic effect in oral SCC. However, neither HPV infection nor p53 mutation influenced survival.

摘要

本研究旨在调查日本患者口腔鳞状细胞癌（SCC）中人乳头瘤病毒（HPV）16型和18型感染以及p53突变的发生率。我们的结果显示，HPV16和18感染的发生率高于以往研究，因为我们综合了共识聚合酶链反应（PCR）、通过对PCR产物进行限制性酶切的限制性片段长度多态性分析以及Southern印迹杂交的结果。在46个样本中，分别有9个（20%）和25个（54%）检测到HPV16和18 E6/E7 DNA。通过PCR-单链构象多态性分析，在46个样本中的20个（43%）检测到外显子5至8中的p53突变。HPV16与p53突变之间存在显著相关性（P = 0.02），表明HPV16感染在口腔SCC中具有诱变作用。然而，HPV感染和p53突变均不影响生存率。