对属于巨孢囊霉科的丛枝菌根真菌中细菌内共生体的检测与鉴定。
Detection and identification of bacterial endosymbionts in arbuscular mycorrhizal fungi belonging to the family Gigasporaceae.
作者信息
Bianciotto V, Lumini E, Lanfranco L, Minerdi D, Bonfante P, Perotto S
机构信息
Centro Studio Micologia del Terreno-CNR, 10125 Turin, Italy.
出版信息
Appl Environ Microbiol. 2000 Oct;66(10):4503-9. doi: 10.1128/AEM.66.10.4503-4509.2000.
Abstract
Intracellular bacteria have been found previously in one isolate of the arbuscular mycorrhizal (AM) fungus Gigaspora margarita BEG 34. In this study, we extended our investigation to 11 fungal isolates obtained from different geographic areas and belonging to six different species of the family Gigasporaceae. With the exception of Gigaspora rosea, isolates of all of the AM species harbored bacteria, and their DNA could be PCR amplified with universal bacterial primers. Primers specific for the endosymbiotic bacteria of BEG 34 could also amplify spore DNA from four species. These specific primers were successfully used as probes for in situ hybridization of endobacteria in G. margarita spores. Neighbor-joining analysis of the 16S ribosomal DNA sequences obtained from isolates of Scutellospora persica, Scutellospora castanea, and G. margarita revealed a single, strongly supported branch nested in the genus Burkholderia.
摘要
此前在丛枝菌根（AM）真菌巨孢囊霉Gigaspora margarita BEG 34的一个分离株中发现了细胞内细菌。在本研究中，我们将调查范围扩大到从不同地理区域获得的11个真菌分离株，它们属于巨孢囊霉科的6个不同物种。除了玫瑰巨孢囊霉外，所有AM物种的分离株都含有细菌，并且它们的DNA可用通用细菌引物进行PCR扩增。针对BEG 34内共生细菌的特异性引物也能从4个物种的孢子DNA中扩增出产物。这些特异性引物成功用作玛格丽特巨孢囊霉孢子内细菌原位杂交的探针。对从波斯盾巨孢囊霉、栗色盾巨孢囊霉和玛格丽特巨孢囊霉分离株获得的16S核糖体DNA序列进行邻接法分析，结果显示在伯克霍尔德菌属中有一个得到强烈支持的单分支。
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