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来自波兰、日本和美国的8种(弗氏)库姆氏菌菌株相关细菌的生物多样性。

Biodiversity of Bacteria Associated with Eight (Fr.) P. Kumm. Strains from Poland, Japan and the USA.

作者信息

Adamski Mariusz, Pietr Stanislaw J

机构信息

Agricultural Microbiology Lab , Department of Plant Protection , The Faculty of Life Sciences and Technology , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.

出版信息

Pol J Microbiol. 2019;68(1):71-81. doi: 10.21307/pjm-2019-009.

Abstract

Few publications report the occurrence of bacteria associated with fungal cells. The presence of bacteria associated with one strain of (Fr.) P. Kumm. was described in the literature. We describe the biodiversity of bacteria associated with eight oyster mushroom strains from Japan, Poland, and the USA. The presence of microorganisms associated with all tested strains was confirmed using fluorescent microscopy. Among 307 sequences, 233 of clones representing 34 genera and 74 sequences were identified as . Most of the bacteria associated with the strain PUSAS were related to and two clones were related to genus. The biodiversity of clones isolated from fungal strains originating from Japan and Poland ranged from 15 to 32 different bacterial clones. The most often the bacteria related to genus , and were associated with the strains of fungi mentioned above. Laccase-like (LMCO) genes were identified in whole bacterial DNA isolated from the associated bacteria but β-glucosidase and β-xylanase genes were not detected. Few publications report the occurrence of bacteria associated with fungal cells. The presence of bacteria associated with one strain of (Fr.) P. Kumm. was described in the literature. We describe the biodiversity of bacteria associated with eight oyster mushroom strains from Japan, Poland, and the USA. The presence of microorganisms associated with all tested strains was confirmed using fluorescent microscopy. Among 307 sequences, 233 of clones representing 34 genera and 74 sequences were identified as . Most of the bacteria associated with the strain PUSAS were related to and two clones were related to genus. The biodiversity of clones isolated from fungal strains originating from Japan and Poland ranged from 15 to 32 different bacterial clones. The most often the bacteria related to genus , and were associated with the strains of fungi mentioned above. Laccase-like (LMCO) genes were identified in whole bacterial DNA isolated from the associated bacteria but β-glucosidase and β-xylanase genes were not detected.

摘要

很少有出版物报道与真菌细胞相关的细菌的存在情况。文献中描述了与一种(Fr.)P. Kumm.菌株相关的细菌的存在。我们描述了来自日本、波兰和美国的8种平菇菌株相关细菌的生物多样性。使用荧光显微镜确认了与所有测试的平菇菌株相关的微生物的存在。在307个序列中,代表34个属的233个克隆序列和74个序列被鉴定为……与PUSAS菌株相关的大多数细菌与……有关,两个克隆与……属有关。从源自日本和波兰的真菌菌株中分离出的克隆的生物多样性范围为15至32个不同的细菌克隆。最常与上述真菌菌株相关的是与……属和……属相关的细菌。在从相关细菌中分离出的全细菌DNA中鉴定出了漆酶样(LMCO)基因,但未检测到β-葡萄糖苷酶和β-木聚糖酶基因。很少有出版物报道与真菌细胞相关的细菌的存在情况。文献中描述了与一种(Fr.)P. Kumm.菌株相关的细菌的存在。我们描述了来自日本、波兰和美国的8种平菇菌株相关细菌的生物多样性。使用荧光显微镜确认了与所有测试的平菇菌株相关的微生物的存在。在307个序列中,代表34个属的233个克隆序列和74个序列被鉴定为……与PUSAS菌株相关的大多数细菌与……有关,两个克隆与……属有关。从源自日本和波兰的真菌菌株中分离出的克隆的生物多样性范围为15至32个不同的细菌克隆。最常与上述真菌菌株相关的是与……属和……属相关的细菌。在从相关细菌中分离出的全细菌DNA中鉴定出了漆酶样(LMCO)基因,但未检测到β-葡萄糖苷酶和β-木聚糖酶基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c2/7256699/851c9e344670/pjm-68-1-071-g001.jpg

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