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本文引用的文献

1
Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes.利用粪便厌氧菌的宿主特异性16S核糖体DNA遗传标记物识别沿海水域粪便污染的非点源。
Appl Environ Microbiol. 2000 Apr;66(4):1587-94. doi: 10.1128/AEM.66.4.1587-1594.2000.
2
Determining sources of fecal pollution in a rural Virginia watershed with antibiotic resistance patterns in fecal streptococci.利用粪链球菌中的抗生素耐药模式确定弗吉尼亚州一个乡村流域的粪便污染源。
Appl Environ Microbiol. 1999 Dec;65(12):5522-31. doi: 10.1128/AEM.65.12.5522-5531.1999.
3
Use of antibiotic resistance analysis to identify nonpoint sources of fecal pollution.利用抗生素抗性分析来识别粪便污染的非点源。
Appl Environ Microbiol. 1999 Aug;65(8):3483-6. doi: 10.1128/AEM.65.8.3483-3486.1999.
4
Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity.通过测量小亚基rRNA基因PCR扩增子长度异质性获得的沿海微微型浮游生物群落结构估计中的动力学偏差。
Appl Environ Microbiol. 1998 Nov;64(11):4522-9. doi: 10.1128/AEM.64.11.4522-4529.1998.
5
Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes.使用针对特定菌群的16S rRNA靶向寡核苷酸探针通过荧光原位杂交技术检测人类粪便中细菌群体的变化。
Appl Environ Microbiol. 1998 Sep;64(9):3336-45. doi: 10.1128/AEM.64.9.3336-3345.1998.
6
Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.通过测定编码16S rRNA的基因的末端限制性片段长度多态性来表征微生物多样性。
Appl Environ Microbiol. 1997 Nov;63(11):4516-22. doi: 10.1128/aem.63.11.4516-4522.1997.
7
Discriminant analysis of antibiotic resistance patterns in fecal streptococci, a method to differentiate human and animal sources of fecal pollution in natural waters.粪便链球菌抗生素耐药模式的判别分析，一种区分天然水体中粪便污染的人类和动物来源的方法。
Appl Environ Microbiol. 1996 Nov;62(11):3997-4002. doi: 10.1128/aem.62.11.3997-4002.1996.
8
Design and evaluation of Bacteroides DNA probes for the specific detection of human fecal pollution.用于特异性检测人类粪便污染的拟杆菌属DNA探针的设计与评估。
Appl Environ Microbiol. 1995 Apr;61(4):1171-9. doi: 10.1128/aem.61.4.1171-1179.1995.
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The Ribosomal Database Project.核糖体数据库项目
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10
Survival and detection of Bacteroides spp., prospective indicator bacteria.拟杆菌属的存活与检测，潜在指示菌
Appl Environ Microbiol. 1985 Jan;49(1):148-50. doi: 10.1128/aem.49.1.148-150.1985.

一种基于编码16S rRNA的拟杆菌-普雷沃氏菌属基因中的宿主差异来区分人类和反刍动物粪便的聚合酶链反应（PCR）检测方法。

A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

作者信息

Bernhard A E, Field K G

机构信息

Department of Microbiology, Oregon State University, Corvallis, Oregon 97330, USA.

出版信息

Appl Environ Microbiol. 2000 Oct;66(10):4571-4. doi: 10.1128/AEM.66.10.4571-4574.2000.

DOI:10.1128/AEM.66.10.4571-4574.2000
PMID:11010920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92346/
Abstract

Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distinguish cow from human feces. Here, we recovered 16S rDNA clones from natural waters that were close phylogenetic relatives of the markers. From the sequence data, we designed specific PCR primers that discriminate human and ruminant sources of fecal contamination.

摘要

我们的目的是开发一种快速、廉价的方法来诊断水中粪便污染的来源。在先前的研究中，我们通过分析确定了拟杆菌-普雷沃氏菌核糖体DNA（rDNA）PCR标记。这些标记通过长度异质性PCR和末端限制性片段长度多态性来区分牛粪和人粪。在这里，我们从天然水中回收了与这些标记具有密切系统发育关系的16S rDNA克隆。根据序列数据，我们设计了特异性PCR引物，以区分人类和反刍动物粪便污染的来源。