一种基于编码16S rRNA的拟杆菌-普雷沃氏菌属基因中的宿主差异来区分人类和反刍动物粪便的聚合酶链反应（PCR）检测方法。
A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.
作者信息
Bernhard A E, Field K G
机构信息
Department of Microbiology, Oregon State University, Corvallis, Oregon 97330, USA.
出版信息
Appl Environ Microbiol. 2000 Oct;66(10):4571-4. doi: 10.1128/AEM.66.10.4571-4574.2000.
Abstract
Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distinguish cow from human feces. Here, we recovered 16S rDNA clones from natural waters that were close phylogenetic relatives of the markers. From the sequence data, we designed specific PCR primers that discriminate human and ruminant sources of fecal contamination.
摘要
我们的目的是开发一种快速、廉价的方法来诊断水中粪便污染的来源。在先前的研究中，我们通过分析确定了拟杆菌-普雷沃氏菌核糖体DNA（rDNA）PCR标记。这些标记通过长度异质性PCR和末端限制性片段长度多态性来区分牛粪和人粪。在这里，我们从天然水中回收了与这些标记具有密切系统发育关系的16S rDNA克隆。根据序列数据，我们设计了特异性PCR引物，以区分人类和反刍动物粪便污染的来源。
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