构巢曲霉creA基因的反义沉默
Antisense silencing of the creA gene in Aspergillus nidulans.
作者信息
Bautista L F, Aleksenko A, Hentzer M, Santerre-Henriksen A, Nielsen J
机构信息
Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, 2800 Lyngby, Denmark.
出版信息
Appl Environ Microbiol. 2000 Oct;66(10):4579-81. doi: 10.1128/AEM.66.10.4579-4581.2000.
Abstract
Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.
摘要
在构巢曲霉中，对编码主要碳分解代谢物阻遏蛋白CREA的基因的一部分进行反义表达，结果发现在有葡萄糖存在的情况下，内源性和异源的葡萄糖可阻遏酶的水平大幅增加。这种去阻遏效应约为creA基因缺失突变体所产生效应的一半。然而，与该突变体的结果不同，反义转化体的生长参数和菌落形态并未受到影响。
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