Nellen W, Lichtenstein C
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Trends Biochem Sci. 1993 Nov;18(11):419-23. doi: 10.1016/0968-0004(93)90137-c.
Antisense RNA has been used for some time as a versatile tool for silencing gene expression. There is ample evidence for gene regulation by endogenous antisense transcripts in prokaryotes and increasing insight into the molecular mechanisms underlying such regulation. The introduction of antisense gene constructs into eukaryotes has now become routine but the mechanisms by which gene expression is inhibited are barely understood. In recent years, several examples of endogenous eukaryotic antisense transcripts have been discovered, some of which probably serve regulatory functions. Here we will discuss a model to explain mechanisms of antisense-mediated gene silencing.
反义RNA作为一种用于沉默基因表达的通用工具已经使用了一段时间。有充分的证据表明原核生物中内源性反义转录本可进行基因调控，并且人们对这种调控背后的分子机制有了越来越深入的了解。现在，将反义基因构建体导入真核生物已成为常规操作，但基因表达受抑制的机制却几乎不为人知。近年来，已经发现了几个内源性真核生物反义转录本的例子，其中一些可能具有调控功能。在这里，我们将讨论一个模型来解释反义介导的基因沉默机制。
