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[血液甘油三酯测定中提取过程的自动化]

[Automation of extraction in the determination of blood triglyceride].

作者信息

Weill J, Bardos P, Collin B

出版信息

Pathol Biol (Paris). 1975 Oct;23(8):657-63.

PMID:1101165
Abstract

A complete automation of the determination of triglycerides is described which replaces the manual extraction and the final determination with an autoanalyzer chain, based on the method of Royer et al. For the automation of triglyceride extraction, we have modified the sampler, by incorporating a magnetic stirrer under the sample table. The sample table has two rows of holes. The sera, which are taken from the inner row and diluted with sulphuric acid, are reacted quantitatively with the extraction reagent in the beakers of the outer row, which contain a magnetic follower. After stirring, the upper phase is quantitatively removed and the determination is started. The problem of phasing the individual steps of the analysis is thus solved.

摘要

本文描述了一种甘油三酯测定的完全自动化方法,该方法基于罗耶等人的方法,用自动分析仪链取代了手动提取和最终测定步骤。为实现甘油三酯提取的自动化,我们对进样器进行了改进,在样品台下方安装了一个磁力搅拌器。样品台有两排孔。从内排孔吸取的血清用硫酸稀释后,与外排孔烧杯中含有磁性搅拌子的提取试剂进行定量反应。搅拌后,定量移去上层液相并开始测定。这样就解决了分析中各个步骤的同步问题。

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