Leplaideur F
Ann Biol Clin (Paris). 1976;34(1):33-40.
The author describes a simple and entirely automatic technique for the estimation of triglycerides in human serum which, in acetic medium, are extracted by a mixture of nonane-isopropanol and saponified. The glycerol is oxidised into formaldehyde which is estimated by fluorimetry after Hantzsch' reaction. Successive stages are discussed together with the precision of the method. The results are in agreement with those obtained by Kessler and Lederer's method.
作者描述了一种简单且完全自动化的技术,用于测定人血清中的甘油三酯。在乙酸介质中,甘油三酯用壬烷 - 异丙醇混合物萃取并皂化。甘油被氧化成甲醛,经汉奇反应后用荧光法测定。文中讨论了该方法的连续步骤及其精密度。结果与凯斯勒和莱德勒方法所得结果一致。