Parsa A T, Chakrabarti I, Hurley P T, Chi J H, Hall J S, Kaiser M G, Bruce J N
Department of Neurological Surgery, The Neurological Institute of New York College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
Neurosurgery. 2000 Oct;47(4):993-9; discussion 999-1000. doi: 10.1097/00006123-200010000-00050.
Intracranial rat glioma models are a useful method for evaluating the efficacy and toxicity of novel therapies for malignant glioma. The C6/Wistar model has been used extensively as a reproducible in vivo model for studying primary brain tumors including anti-glioma immune responses. The objective of the present study is to provide in vivo evidence that the C6 rat glioma model is allogeneic within Wistar rats and is therefore inappropriate for evaluating immune responses.
Growth patterns and immune responses of C6 cells implanted into the brain and flank of Wistar rats were analyzed and compared to an immunogenic syngeneic model (9L/Fischer).
Wistar rats with C6 tumors developed a potent humoral and cellular immune response to the tumor. Wistar rats given simultaneous flank and intracerebral tumors had a survival rate of 100% compared to an 11% survival rate in control animals receiving only intracranial C6 cells.
The C6 rat glioma induces a vigorous immune reaction that may mimic a specific anti-tumor response in Wistar rats. Efficacy of immunotherapy within this model must be cautiously interpreted.
颅内大鼠胶质瘤模型是评估恶性胶质瘤新疗法疗效和毒性的一种有用方法。C6/Wistar模型已被广泛用作研究原发性脑肿瘤（包括抗胶质瘤免疫反应）的可重复体内模型。本研究的目的是提供体内证据，证明C6大鼠胶质瘤模型在Wistar大鼠体内是同种异体的，因此不适用于评估免疫反应。
分析植入Wistar大鼠脑内和侧腹的C6细胞的生长模式和免疫反应，并与免疫原性同基因模型（9L/费希尔大鼠）进行比较。
患有C6肿瘤的Wistar大鼠对肿瘤产生了强烈的体液和细胞免疫反应。同时接种侧腹和脑内肿瘤的Wistar大鼠存活率为100%，而仅接受颅内C6细胞的对照动物存活率为11%。
C6大鼠胶质瘤可诱导强烈的免疫反应，这可能模拟Wistar大鼠体内特定的抗肿瘤反应。在此模型中免疫疗法的疗效必须谨慎解读。
