Koo H, Vacca Smith A M, Bowen W H, Rosalen P L, Cury J A, Park Y K
Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, Brazil.
Caries Res. 2000 Sep-Oct;34(5):418-26. doi: 10.1159/000016617.
Propolis, a resinous hive product collected by Apis mellifera bees, has been used for thousands of years in folk medicine. Ethanolic extracts of propolis (EEP) have been shown to inhibit the activity of a mixture of crude glucosyltransferase (Gtf) enzymes in solution. These enzymes synthesize glucans from sucrose, which are important for the formation of pathogenic dental plaque. In the present study, the effects of propolis from two different regions of Brazil on the activity of separate, purified Gtf enzymes in solution and on the surface of saliva-coated hydroxyapatite (sHA) beads were evaluated. The EEP from Minas Gerais (MG; Southeastern Brazil) and Rio Grande do Sul (RS; Southern Brazil) were tested for their ability to inhibit the enzymes GtfB (synthesis of insoluble glucan), GtfC (insoluble/soluble glucan) and GtfD (soluble glucan). The effects of propolis on Gtf from Streptococcus sanguis (soluble glucan synthesis) was also explored. The EEP from both regions effectively inhibited the activity of all Gtfs in solution (75-95%) and on the surface of sHA beads (45-95%) at concentrations between 0.75 and 3.0 mg of propolis/ml. However, the two samples of propolis showed different levels of inhibition on each of the enzymes tested. In general, EEP RS demonstrated a significantly higher inhibitory activity on GtfB and C activities (both solution and surface assays) than EEP MG at concentrations between 0.047 and 0.187 mg/ml (p<0.05). EEP MG, on the other hand, exhibited a greater inhibitory effect on the activities of surface GtfD (at 0.375, 0.75 and 1.5 mg/ml) and S. sanguis Gtf (at 1.5 and 3.0 mg/ml; p<0.05). These data indicate that EEP is a potent inhibitor of Gtf enzymes in solution and adsorbed on an experimental pellicle; however, its effect on Gtf activity is variable depending on the geographical origin of the propolis samples. There is a need to identify the active compounds of propolis.
蜂胶是蜜蜂采集的一种树脂类蜂巢产品，在民间医学中已使用了数千年。蜂胶乙醇提取物（EEP）已被证明能抑制溶液中粗制葡糖基转移酶（Gtf）混合物的活性。这些酶从蔗糖合成葡聚糖，而葡聚糖对致病性牙菌斑的形成很重要。在本研究中，评估了来自巴西两个不同地区的蜂胶对溶液中单独纯化的Gtf酶活性以及对唾液包被的羟基磷灰石（sHA）珠表面的影响。测试了来自米纳斯吉拉斯州（MG；巴西东南部）和南里奥格兰德州（RS；巴西南部）的EEP抑制GtfB（不溶性葡聚糖合成）、GtfC（不溶性/可溶性葡聚糖）和GtfD（可溶性葡聚糖）酶的能力。还探讨了蜂胶对血链球菌Gtf（可溶性葡聚糖合成）的影响。来自两个地区的EEP在蜂胶浓度为0.75至3.0毫克/毫升时，均能有效抑制溶液中所有Gtf的活性（75 - 95%）以及sHA珠表面的活性（45 - 95%）。然而，这两种蜂胶样品对每种测试酶的抑制水平不同。总体而言，在浓度为0.047至0.187毫克/毫升时，EEP RS对GtfB和C活性（溶液和表面测定）的抑制活性明显高于EEP MG（p<0.05）。另一方面，EEP MG对表面GtfD活性（在0.375、0.75和1.5毫克/毫升时）和血链球菌Gtf活性（在1.5和3.0毫克/毫升时；p<0.05）表现出更大的抑制作用。这些数据表明，EEP是溶液中以及吸附在实验性薄膜上的Gtf酶的有效抑制剂；然而，其对Gtf活性的影响因蜂胶样品的地理来源而异。有必要鉴定蜂胶的活性成分。
