Koo H, Rosalen P L, Cury J A, Park Y K, Ikegaki M, Sattler A
Department of Physiological Sciences, Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, Brazil.
Caries Res. 1999 Sep-Oct;33(5):393-400. doi: 10.1159/000016539.
The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of Brazil on caries development in desalivated rats. Ethanolic extracts of propolis (EEP) were prepared from crude propolis samples collected in Minas Gerais state (MG), southeastern Brazil, and Rio Grande do Sul state (RS), southern Brazil. The flavonoid composition of EEP was analyzed by high-performance thin-layer chromatography (HPTLC) and reversed-phase high-performance liquid chromatography (HPLC). For the animal study, 30 specific pathogen-free Wistar rats were infected with Streptococcus sobrinus 6715 and surgically desalivated. The rats were randomly divided into three groups which were treated with 80% ethanol (control), EEP from MG and EEP from RS. The animals were placed in a König-Höfer programmed feeder and received 17 meals of diet 2000 daily at hourly intervals. The solutions were applied on the rat molars (25 microl on molars of each quadrant) twice a day, by using graduate syringes. After 3 weeks, the animals were killed by CO(2) asphyxiation. For microbial assessment, the left jaw was removed and sonicated in 154 mM NaCl solution. Dental caries was evaluated according to Larson's modification of Keyes' system. The HPTLC patterns and HPLC profiles demonstrated that both quality and quantity of flavonoid aglycones of EEP from MG were different compared to EEP from RS. In general, it is apparent that EEP from RS contained the highest concentrations of pinocembrin, chrysin, acacetin and galangin. The group of animals treated with EEP from RS showed the lowest smooth-surface and sulcal caries scores as well as less caries severity in smooth-surface and sulcal lesions, and these data were statistically different when compared with the control group. The group treated with EEP from MG only demonstrated a significant difference in the severity of sulcal lesions when compared to the control group. The percentage of S. sobrinus was lower in the groups treated with EEP, but did not differ statistically from the control group. The results showed that the cariostatic effect of propolis depends on its composition, and consequently the region of collection of propolis samples.
本研究的目的是评估从巴西两个地区采集的蜂胶对去唾液大鼠龋齿发展的影响。蜂胶乙醇提取物（EEP）由从巴西东南部米纳斯吉拉斯州（MG）和巴西南部南里奥格兰德州（RS）采集的粗蜂胶样品制备而成。通过高效薄层色谱法（HPTLC）和反相高效液相色谱法（HPLC）分析EEP的黄酮类成分。在动物研究中，30只无特定病原体的Wistar大鼠感染了远缘链球菌6715并通过手术去除唾液。大鼠被随机分为三组，分别用80%乙醇（对照组）、来自MG的EEP和来自RS的EEP进行处理。将动物置于König-Höfer程控喂食器中，每天每隔一小时接受17餐2000号饮食。使用刻度注射器每天两次将溶液涂覆在大鼠磨牙上（每个象限的磨牙上涂25微升）。3周后，通过二氧化碳窒息法处死动物。为了进行微生物评估，取下左颌并在154 mM氯化钠溶液中超声处理。根据Larson对Keyes系统的改良评估龋齿情况。HPTLC图谱和HPLC谱表明，与来自RS的EEP相比，来自MG的EEP中黄酮苷元的质量和数量均有所不同。总体而言，显然来自RS的EEP中松属素、白杨素、刺槐素和高良姜素的浓度最高。用来自RS的EEP处理的动物组的平滑面和沟裂龋得分最低，平滑面和沟裂病变的龋齿严重程度也较低，与对照组相比，这些数据具有统计学差异。与对照组相比，用来自MG的EEP处理的组仅在沟裂病变严重程度上表现出显著差异。用EEP处理的组中远缘链球菌的百分比较低，但与对照组相比无统计学差异。结果表明，蜂胶的抑龋作用取决于其成分，因此也取决于蜂胶样品的采集地区。
